Lysobacter arvi sp. nov. Isolated from Farmland Soil

• Published in: Current microbiology 2023-12, Vol.80 (12), p.387-387, Article 387
• Main Authors: Choksket, Stanzin, Harshvardhan, Rana, Rekha, Patil, Prabhu B., Korpole, Suresh
• Format: Article
• Language: English
• Subjects: Agricultural land; Bacteria; Biomedical and Life Sciences; Biotechnology; Cardiolipin; Cell morphology; Cytology; Deoxyribonucleic acid; DNA; Fatty acids; Genomes; Genomic analysis; Hybridization; Life Sciences; Lipids; Lysobacter; Methods; Microbiology; Morphology; New species; Nucleotide sequence; Nucleotides; Phosphatidylethanolamine; Phosphatidylglycerol; Phylogenetics; Phylogeny; Quinones; rRNA 16S; Sequence analysis; Similarity; Soil microorganisms; Soils; Strain analysis; Strains (organisms); Taxonomy; Topology
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-023-03486-8

A bacterial strain designated as UC was isolated from farmland soil. Strain UC T formed a pale yellow colony on nutrient agar. Cell morphology revealed it as the rod-shaped bacterium that stained Gram-negative. The 16S rRNA gene sequence analysis identified strain UC T as a member of the genus Lysobacter that showed high identity with L. soli DCY21 T (99.5%), L. panacisoli CJ29 T (98.7%), and L. tabacisoli C8-1 T (97.9%). It formed a distinct cluster with these strains in the neighbor-joining phylogenetic tree. A similar tree topology was observed in TYGS-based phylogenomic analysis. However, genome sequence analyses of strain UC T showed 87.7% average nucleotide identity and 34.7% digital DNA–DNA hybridization similarity with the phylogenetically closest species, L. soli DCY21 T . The similarity was much less with other closely related strains of the genus Lysobacter . The G + C content of strain UC T was 68.1%. Major cellular fatty acids observed were C 14:0 iso (13.4%), C 15:0 iso (13.6%), and C 15:0 anteiso (14.8%). Quinone Q-8 was the major respiratory ubiquinone. Predominant polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol. Production of xanthomonadin pigment was observed. Based on phenotypic differences and phylogenomic analysis, strain UC T represents a novel species of the genus Lysobacter , for which the name Lysobacter arvi is proposed. The type strain of the novel species is UC T (= KCTC 92613 T  = JCM 23757 T  = MTCC 12824 T ).