Proteome insights of citric acid-mediated cadmium toxicity tolerance in Brassica napus L

• Published in: Environmental science and pollution research international 2023-11, Vol.30 (54), p.115461-115479
• Main Authors: Mittra, Probir Kumar, Roy, Swapan Kumar, Rahman, Md Atikur, Naimuzzaman, Mollah, Kwon, Soo-Jeong, Yun, Sung Ho, Cho, Kun, Katsube-Tanaka, Tomoyuki, Shiraiwa, Tatsuhiko, Woo, Sun-Hee
• Format: Article
• Language: English
• Subjects: Adenosylmethionine; Antioxidants; Aquatic Pollution; Ascorbic acid; Assimilation; Atmospheric Protection/Air Quality Control/Air Pollution; Biological activity; Brassica; Cadmium; Carbohydrate metabolism; Carbohydrates; Carbon dioxide; Cell walls; Citric acid; Crop production; Cysteine synthase; Cytoskeleton; Detoxification; Dietary supplements; Earth and Environmental Science; Ecotoxicology; Energy metabolism; Environment; Environmental Chemistry; Environmental Health; Food chains; Food production; Food security; Glutamate-ammonia ligase; Glutamine; Glutathione; Glutathione transferase; Heavy metals; L-Ascorbate peroxidase; Metabolism; Oilseed crops; Peroxidase; Photosynthesis; Plant breeding; Protein metabolism; Protein turnover; Proteins; Research Article; Signal transduction; Sulfur; Superoxide dismutase; Toxic substances; Toxicity; Toxicity tolerance; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 1614-7499; 0944-1344; 1614-7499
• DOI: 10.1007/s11356-023-30442-7

Cadmium (Cd) is a toxic substance that is uptake by plants from soils, Cd easily transfers into the food chain. Considering global food security, eco-friendly, cost-effective, and metal detoxification strategies are highly demandable for sustainable food crop production. The purpose of this study was to investigate how citric acid (CA) alleviates or tolerates Cd toxicity in Brassica using a proteome approach. In this study, the global proteome level was significantly altered under Cd toxicity with or without CA supplementation in Brassica . A total of 4947 proteins were identified using the gel-free proteome approach. Out of these, 476 proteins showed differential abundance between the treatment groups, wherein 316 were upregulated and 160 were downregulated. The gene ontology analysis reveals that differentially abundant proteins were involved in different biological processes including energy and carbohydrate metabolism, CO 2 assimilation and photosynthesis, signal transduction and protein metabolism, antioxidant defense, heavy metal detoxification, plant development, and cytoskeleton and cell wall structure in Brassica leaves. Interestingly, several candidate proteins such as superoxide dismutase (A0A078GZ68) l -ascorbate peroxidase 3 (A0A078HSG4), glutamine synthetase (A0A078HLB2), glutathione S -transferase DHAR1 (A0A078HPN8), glutamine synthetase (A0A078HLB2), cysteine synthase (A0A078GAD3), S -adenosylmethionine synthase 2 (A0A078JDL6), and thiosulfate/3-mercaptopyruvate sulfur transferase 2 (A0A078H905) were involved in antioxidant defense system and sulfur assimilation-involving Cd-detoxification process in Brassica . These findings provide new proteome insights into CA-mediated Cd-toxicity alleviation in Brassica , which might be useful to oilseed crop breeders for enhancing heavy metal tolerance in Brassica using the breeding program, with sustainable and smart Brassica production in a metal-toxic environment.