LSM14B is essential for oocyte meiotic maturation by regulating maternal mRNA storage and clearance

Abstract Fully grown oocytes remain transcriptionally quiescent, yet many maternal mRNAs are synthesized and retained in growing oocytes. We now know that maternal mRNAs are stored in a structure called the mitochondria-associated ribonucleoprotein domain (MARDO). However, the components and functio...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 2023-11, Vol.51 (21), p.11652-11667
Main Authors: Wan, Yanling, Yang, Shuang, Li, Tongtong, Cai, Yuling, Wu, Xinyue, Zhang, Mingyu, Muhammad, Tahir, Huang, Tao, Lv, Yue, Chan, Wai-Yee, Lu, Gang, Li, Jingxin, Sha, Qian-Qian, Chen, Zi-Jiang, Liu, Hongbin
Format: Article
Language:English
Subjects:
Animals
Female
Humans
Male
Meiosis - genetics
Mice
Mice, Inbred C57BL
Oocytes - physiology
Oogenesis - genetics
RNA, Messenger - genetics
RNA, Messenger, Stored - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Fully grown oocytes remain transcriptionally quiescent, yet many maternal mRNAs are synthesized and retained in growing oocytes. We now know that maternal mRNAs are stored in a structure called the mitochondria-associated ribonucleoprotein domain (MARDO). However, the components and functions of MARDO remain elusive. Here, we found that LSM14B knockout prevents the proper storage and timely clearance of mRNAs (including Cyclin B1, Btg4 and other mRNAs that are translationally activated during meiotic maturation), specifically by disrupting MARDO assembly during oocyte growth and meiotic maturation. With decreased levels of storage and clearance, the LSM14B knockout oocytes failed to enter meiosis II, ultimately resulting in female infertility. Our results demonstrate the function of LSM14B in MARDO assembly, and couple the MARDO with mRNA clearance and oocyte meiotic maturation. Graphical Abstract Graphical Abstract
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/gkad919