A Colorimetric Biosensing Platform with Aptamers, Rolling Circle Amplification and Urease‐Mediated Litmus Test

Here we report on an ultra‐sensitive colorimetric sensing platform that takes advantage of both the strong amplification power of rolling circle amplification (RCA) and the high efficiency of a simple urease‐mediated litmus test. The presence of a target triggers the RCA reaction, and urease‐labelle...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2023-12, Vol.62 (51), p.e202315185-n/a
Main Authors: Chang, Dingran, Li, Jiuxing, Liu, Rudi, Liu, Meng, Tram, Kha, Schmitt, Natalie, Li, Yingfu
Format: Article
Language:English
Subjects:
Amplification
Aptamer
Aptamers
Aptamers, Nucleotide
Biosensing
Biosensing Techniques
Biosensors
Colorimetry
Deoxyribonucleic acid
DNA
DNA - metabolism
Growth factors
Humans
Litmus Test
Nucleic Acid Amplification Techniques
Platelet-derived growth factor
Rolling Circle Amplification
Saliva
Severe acute respiratory syndrome coronavirus 2
Streptavidin
Target detection
Thrombin
Urea
Urease
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Summary:Here we report on an ultra‐sensitive colorimetric sensing platform that takes advantage of both the strong amplification power of rolling circle amplification (RCA) and the high efficiency of a simple urease‐mediated litmus test. The presence of a target triggers the RCA reaction, and urease‐labelled DNA can hybridize to the biotinylated RCA products and be immobilized onto streptavidin‐coated magnetic beads. The urease‐laden beads are then used to hydrolyze urea, leading to an increase in pH that can be detected by a simple litmus test. We show this sensing platform can be easily integrated with aptamers for sensing diverse targets via the detection of human thrombin and platelet‐derived growth factor (PDGF) utilizing structure‐switching aptamers as well as SARS‐CoV‐2 in human saliva using a spike‐binding trimeric DNA aptamer. Furthermore, we demonstrate that this colorimetric sensing platform can be integrated into a simple paper‐based device for sensing applications. Sensitive and simple‐to‐use colorimetric sensors are highly desirable for point‐of‐care diagnostic applications. Here we report on an ultra‐sensitive colorimetric sensing platform that takes advantage of both the strong amplification power of rolling circle amplification and the high efficiency of a urease‐mediated litmus test. By using aptamers for target recognition, this platform can be adapted for a wide range of sensing applications.
ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.202315185