Biophysical and biochemical characterization of the thioredoxin system from Colwellia psychrerythraea

The thioredoxin system is a ubiquitous oxidoreductase system consisting of the enzyme thioredoxin reductase, the protein thioredoxin, and the cofactor nicotinamide adenine dinucleotide phosphate. The system has been comprehensively studied from many organisms, such as Escherichia coli; however, stru...

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Published in:Proteins, structure, function, and bioinformatics structure, function, and bioinformatics, 2024-03, Vol.92 (3), p.370-383
Main Authors: Sahtout, Naheda, Sanders, David A R
Format: Article
Language:English
Subjects:
Adenine
Bacteria
E coli
Functional analysis
Genomes
NADPH-diaphorase
Nicotinamide
Nicotinamide adenine dinucleotide
Nucleotide sequence
Oxidoreductase
Proteins
Psychrophilic bacteria
Reductases
Structure-function relationships
Thioredoxin
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Summary:The thioredoxin system is a ubiquitous oxidoreductase system consisting of the enzyme thioredoxin reductase, the protein thioredoxin, and the cofactor nicotinamide adenine dinucleotide phosphate. The system has been comprehensively studied from many organisms, such as Escherichia coli; however, structural and functional analysis of this system from psychrophilic bacteria has not been as extensive. In this study, the thioredoxin system proteins of a psychrophilic bacterium, Colwellia psychrerythraea, were characterized using biophysical and biochemical techniques. Analysis of the complete genome sequence of the C. psychrerythraea thioredoxin system suggested the presence of a putative thioredoxin reductase and at least three thioredoxin. In this study, these identified putative thioredoxin system components were cloned, overexpressed, purified, and characterized. Our studies have indicated that the thioredoxin system proteins from E. coli were more stable than those from C. psychrerythraea. Consistent with these results, kinetic assays indicated that the thioredoxin reductase from E. coli had a higher optimal temperature than that from C. psychrerythraea.
ISSN:0887-3585
1097-0134
DOI:10.1002/prot.26627