Evaluation of the ceftazidime/avibactam and meropenem susceptibility by MALDI-TOF MS directly from positive blood cultures

The purpose of this study was to evaluate the MBT-ASTRA to determine susceptibility to ceftazidime/avibactam (CZA) and meropenem (MEM) of Enterobacterales directly from positive blood cultures (BC). Bacterial suspension was incubated with antibiotic and analyzed by MALDI-TOF MS. The relative growth...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2024, Vol.43 (1), p.171-175
Main Authors: Wilhelm, Camila Mörschbächer, Moreira, Natália Kehl, Inamine, Everton, Caierão, Juliana, Barth, Afonso Luís
Format: Article
Language:English
Subjects:
Agreements
Anti-Bacterial Agents - pharmacology
Antibiotics
beta-Lactamases
Biomedical and Life Sciences
Biomedicine
Blood
Blood Culture
Brief Report
Ceftazidime
Ceftazidime - pharmacology
Drug Combinations
E coli
Humans
Infectious diseases
Internal Medicine
Klebsiella
Medical Microbiology
Meropenem
Meropenem - pharmacology
Microbial Sensitivity Tests
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:The purpose of this study was to evaluate the MBT-ASTRA to determine susceptibility to ceftazidime/avibactam (CZA) and meropenem (MEM) of Enterobacterales directly from positive blood cultures (BC). Bacterial suspension was incubated with antibiotic and analyzed by MALDI-TOF MS. The relative growth was calculated and cutoff values were determined to categorize isolates as “S,” “I,” and “R.” Klebsiella spp. with CZA 20/8 mg/L and 1.5-h incubation presented 1 (5.9%) major discrepancy and 96.3% category agreement; other species required 2.5 h for 100% category agreement. For MEM, 4 mg/L and 1.5h were necessary, demonstrating 2 (6.67%) minor discrepancies and 93.3% categorical agreement.
ISSN:0934-9723
1435-4373
DOI:10.1007/s10096-023-04696-5