Effects of aflatoxin B1 on human breast cancer (MCF-7) cells: cytotoxicity, oxidative damage, metabolic, and immune-modulatory transcriptomic changes

Aflatoxin B1 (AFB1) is a potent mycotoxin that is commonly produced by molds such as Aspergillus (A.) flavus and A. parasiticus. AFB1 is associated with several health adverse effects in humans including mutagenesis and carcinogenesis. Aflatoxin is commonly secreted in the milk leading to deleteriou...

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Published in:Environmental science and pollution research international 2023-01, Vol.30 (5), p.13132-13140
Main Authors: Aljazzar, Ahmed, El-Ghareeb, Waleed Rizk, Darwish, Wageh Sobhy, Abdel-Raheem, Sherief M., Ibrahim, Abdelazim M., Hegazy, Eman E., Mohamed, Esraa A.
Format: Article
Language:English
Subjects:
aflatoxin B1
Aflatoxin B1 - metabolism
Aflatoxin B1 - toxicity
Aflatoxins
antioxidants
Aquatic Pollution
Aspergillus
Atmospheric Protection/Air Quality Control/Air Pollution
Breast Neoplasms
breasts
carcinogenesis
cytotoxicity
Earth and Environmental Science
Ecotoxicology
Environment
Environmental Chemistry
Environmental Health
Female
Humans
interleukins
MCF-7 Cells
milk
mutagenesis
necrosis
neoplasm cells
Oxidative Stress
prostaglandin synthase
Research Article
Transcriptome
transcriptomics
Waste Water Technology
Water Management
Water Pollution Control
xenobiotics
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Summary:Aflatoxin B1 (AFB1) is a potent mycotoxin that is commonly produced by molds such as Aspergillus (A.) flavus and A. parasiticus. AFB1 is associated with several health adverse effects in humans including mutagenesis and carcinogenesis. Aflatoxin is commonly secreted in the milk leading to deleterious effects on breast tissue and potential nursing infants. However, the effects of aflatoxins, particularly AFB1, on the breast cells are less investigated. In this study, AFB1-associated effects on human breast cancer cell lines (MCF-7) were investigated. AFB1 caused significant cytotoxicity on MCF-7 cells. Such cytotoxicity had a positive correlation with the induction of oxidative stress. In addition, AFB1 caused significant transcriptomic alterations in xenobiotics and drug-metabolizing enzymes, transporters, and antioxidant enzymes. Besides, AFB1 upregulated pro-inflammatory markers such as tumor necrosis factor-α and cyclooxygenase-2 with a significant reduction of mRNA expressions of the immunity-related genes including interleukins 8 and 10.
ISSN:1614-7499
1614-7499
DOI:10.1007/s11356-022-23032-6