Galactose sensor based on galactose oxidase immobilized in polyvinyl formal

A galactose biosensor was developed by immobilizing galactose oxidase in a polyvinyl formal membrane and attached to the oxygen electrode of a dissolved oxygen analyzer for estimation of galactose in milk and milk products. The immobilized enzyme membrane was characterized by atomic force microscopy...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2006-11, Vol.119 (1), p.15-19
Main Authors: Sharma, Sandeep K., Suman, Pundir, C.S., Sehgal, Neeta, Kumar, Ashok
Format: Article
Language:English
Subjects:
Galactose
Galactose oxidase
Galactose sensor
Galactosemia
Polyvinyl formal
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Summary:A galactose biosensor was developed by immobilizing galactose oxidase in a polyvinyl formal membrane and attached to the oxygen electrode of a dissolved oxygen analyzer for estimation of galactose in milk and milk products. The immobilized enzyme membrane was characterized by atomic force microscopy. The biosensor showed the linearity for 0.5–3.0 g dl −1 galactose. The effects of pH and temperature and the stability of the immobilized galactose oxidase membrane were also studied. The enzyme membrane was found stable at temperatures up to 35 °C and had a shelf life of more than 90 days.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2005.11.046