The impact of Resolvin E1 on bone regeneration in critical‐sized calvarial defects of rat model—A gene expression and micro‐CT analysis

Objective To investigate, in vivo, the effect of local application of Resolvin E1 (RvE1) on the bone regeneration of critical‐size defects (CSDs) in Wistar rats utilizing gene expression and micro‐computed tomographic (micro‐CT) analysis. Background The inflammation‐resolving actions of RvE1 are wel...

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Published in:Journal of periodontal research 2024-02, Vol.59 (1), p.195-203
Main Authors: Alrumaih, Sara, Alshibani, Nouf, Alssum, Lamees, Alshehri, Fahad A., AlMayrifi, Mohammed A., AlMayouf, Mohammed, Alrahlah, Ali, Bautista, Leonel S. J.
Format: Article
Language:English
Subjects:
Alkaline phosphatase
alveolar bone loss
Animal models
Bone grafts
Bone growth
Bone imaging
bone regeneration
Computed tomography
DNA biosynthesis
Eicosapentaenoic acid
Gene expression
Molecular modelling
Osteocalcin
Osteopontin
periodontitis
Regeneration
resolvin
Skin & tissue grafts
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Summary:Objective To investigate, in vivo, the effect of local application of Resolvin E1 (RvE1) on the bone regeneration of critical‐size defects (CSDs) in Wistar rats utilizing gene expression and micro‐computed tomographic (micro‐CT) analysis. Background The inflammation‐resolving actions of RvE1 are well established. The molecular mechanism of its bone‐regenerative actions has been of significant interest in recent years; however, there is limited information regarding the same. Materials and Methods Thirty Wistar rats with a 5 mm induced critical‐size calvarial defect were randomly allocated into four groups: no treatment/negative control (n = 5), treatment using bovine bone grafts/positive control (n = 5), treatment using local delivery of RvE1 (n = 11) and treatment using RvE1 mixed with bovine bone graft (n = 9). After 4 weeks, RNA isolation, complementary DNA synthesis and real‐time polymerase chain reaction were used for genetic expression of alkaline phosphatase (ALP), osteocalcin (OCN) and osteopontin (OPN). The rats were sacrificed after 12 weeks and micro‐CT imaging was performed to analyse the characteristics of the newly formed bone (NFB). The data were analysed using ANOVA and the least significant difference tests (α ≤ .05). Results The RvE1 + bovine graft group had statistically highest mean NFB (20.75 ± 2.67 mm3) compared to other groups (p 
ISSN:0022-3484
1600-0765
DOI:10.1111/jre.13206