Albumin Retains Its Transport Function after Interaction with Cerium Dioxide Nanoparticles

The interaction of inorganic nanomaterials with biological fluids containing proteins can lead not only to the formation of a protein corona and thereby to a change in the biological activity of nanoparticles but also to a significant effect on the structural and functional properties of the biomole...

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Bibliographic Details
Published in:ACS biomaterials science & engineering 2023-12, Vol.9 (12), p.6759-6772
Main Authors: Sozarukova, Madina M., Kochneva, Ekaterina M., Proskurnina, Elena V., Mikheev, Ivan V., Novikov, Dmitry O., Proskurnin, Mikhail A., Ivanov, Vladimir K.
Format: Article
Language:English
Subjects:
Bio-interactions and Biocompatibility
Cerium - chemistry
Cerium - metabolism
Cerium - pharmacology
Humans
Nanoparticles - chemistry
Nanostructures
Serum Albumin, Human - metabolism
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Summary:The interaction of inorganic nanomaterials with biological fluids containing proteins can lead not only to the formation of a protein corona and thereby to a change in the biological activity of nanoparticles but also to a significant effect on the structural and functional properties of the biomolecules themselves. This work studied the interaction of nanoscale CeO2, the most versatile nanozyme, with human serum albumin (HSA). Fourier transform infrared spectroscopy, MALDI-TOF mass spectrometry, UV–vis spectroscopy, and fluorescence spectroscopy confirmed the formation of HSA-CeO2 nanoparticle conjugates. Changes in protein conformation, which depend on the concentration of both citrate-stabilized CeO2 nanoparticles and pristine CeO2 nanoparticles, did not affect albumin drug-binding sites and, accordingly, did not impair the HSA transport function. The results obtained shed light on the biological consequences of the CeO2 nanoparticles’ entrance into the body, which should be taken into account when engineering nanobiomaterials to increase their efficiency and reduce the side effects.
ISSN:2373-9878
2373-9878
DOI:10.1021/acsbiomaterials.3c01416