Fallacies of a purely virtual platform: Virtual plus reality versus virtual reality - A case study

Pretransplant immunological assessment of a transplant donor has evolved significantly over the last few decades with the advent of testing platforms with enhanced sensitivity and varying formats. The single antigen bead assay (SAB) assay, a virtual crossmatch (vXM) is used extensively and considere...

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Bibliographic Details
Published in:Transplant immunology 2023, Vol.81, p.101956-101956
Main Authors: Gnanaraj, John, Doss, Sam Arul, Stephen, S, Pratheeba, M, Daniel, Dolly
Format: Report
Language:English
Online Access:Get full text
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Summary:Pretransplant immunological assessment of a transplant donor has evolved significantly over the last few decades with the advent of testing platforms with enhanced sensitivity and varying formats. The single antigen bead assay (SAB) assay, a virtual crossmatch (vXM) is used extensively and considered the gold standard for defining donor-specific antibodies (DSA) in many parts of the World. A country like India, is however challenged by the lack of adequate representation of locally frequent HLA alleles and hence in our institution, we continue to perform a physical crossmatch (pXM) on the Complement Dependent Cytotoxicity and flow cytometry platforms alongside the SAB. We report here a case report where the discrepancy between platforms of testing have raised certain pertinent questions in our interpretation of the vXM.
ISSN:1878-5492
DOI:10.1016/j.trim.2023.101956