Populus euphratica plant cadmium tolerance PePCR3 improves cadmium tolerance

Abstract Contamination of soils with toxic heavy metals is a major environmental problem. Growing crop plants that can promote the efflux of heavy metals is an effective strategy in contaminated soils. The plant cadmium resistance (PCR) protein is involved in the translocation of heavy metals, speci...

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Bibliographic Details
Published in:Tree physiology 2023-11, Vol.43 (11), p.1950-1963
Main Authors: Qiao, Kun, Shan, Qinghua, Zhang, Haizhen, Lv, Fuling, Zhou, Aimin
Format: Article
Language:English
Subjects:
Biodegradation, Environmental
Cadmium - metabolism
Metals, Heavy - metabolism
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - genetics
Plant Roots - metabolism
Populus - metabolism
Saccharomyces cerevisiae
Soil
Zinc - metabolism
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Summary:Abstract Contamination of soils with toxic heavy metals is a major environmental problem. Growing crop plants that can promote the efflux of heavy metals is an effective strategy in contaminated soils. The plant cadmium resistance (PCR) protein is involved in the translocation of heavy metals, specifically zinc and cadmium (Cd). In this study, yeast expressing Populus euphratica PCR3 (PePCR3) showed enhanced Cd tolerance and decreased Cd accumulation under Cd treatment. Real-time quantitative PCR analyses revealed up-regulation of PePCR3 in poplar seedlings under Cd stress. Localization analysis revealed that PePCR3 localizes at the plasma membrane. The plant growth and biomass were greater in PePCR3-overexpressing (OE) transgenic hybrid poplar lines than in wild type (WT). Physiological parameters analyses indicated that, compared with WT, PePCR3-OE transgenic lines were more tolerant to Cd. In addition, more Cd was excreted in the roots of the PePCR3-OE transgenic lines than in those of WT, but the remaining Cd in transgenic lines was more translocated into the stems and leaves. Eight genes encoding transporters showed increased transcript levels in PePCR3-OE transgenic lines under Cd treatment, implying that PePCR3 interacts with other transporters to translocate Cd. Thus, PePCR3 may be an important genetic resource for generating new lines that can enhance Cd translocation to phytoremediation in contaminated soils.
ISSN:1758-4469
1758-4469
DOI:10.1093/treephys/tpad103