Src Tyrosine Kinase Inhibitory and Antioxidant Activity of Black Chokeberry and Bilberry Fruit Extracts Rich in Chlorogenic Acid

Edible berries such as the fruits of black chokeberry (Aronia melanocarpa (Michx.) Elliott) and bilberry (Vaccinium myrtillus L.) are considered to be rich in phenolic compounds, which are nowadays attracting great interest due to their promising health benefits. The main objective of our study was...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-11, Vol.24 (21), p.15512
Main Authors: Vladimir-Knežević, Sanda, Bival Štefan, Maja, Blažeković, Biljana, Jelić, Dubravko, Petković, Tea, Mandić, Marta, Šprajc, Ekaterina, Lovković, Sandy
Format: Article
Language:English
Subjects:
Antidiabetics
Antioxidants
Berries
Cancer
Chronic diseases
Development and progression
Dietary supplements
Flavonoids
Fruit
Fruits
Homeostasis
Inflammation
Kinases
Lipids
Metabolism
Oxidative stress
Phenols
Phosphorylation
Polyphenols
Proteins
Signal transduction
Tyrosine
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Summary:Edible berries such as the fruits of black chokeberry (Aronia melanocarpa (Michx.) Elliott) and bilberry (Vaccinium myrtillus L.) are considered to be rich in phenolic compounds, which are nowadays attracting great interest due to their promising health benefits. The main objective of our study was to investigate, for the first time, their inhibitory properties on Src tyrosine kinase activity, as this enzyme plays an important role in multiple cellular processes and is activated in both cancer and inflammatory cells. In hydroethanolic fruit extracts, 5.0–5.9% of total polyphenols were determined spectrophotometrically, including high amounts of hydroxycinnamic acid derivatives. HPLC analysis revealed that the black chokeberry and bilberry extracts contained 2.05 mg/g and 2.54 mg/g of chlorogenic acid, respectively. Using a time-resolved fluorescence resonance energy transfer (TR-FRET) assay, the extracts studied were found to have comparable inhibitory effects on Src tyrosine kinase, with IC50 values of 366 µg/mL and 369 µg/mL, respectively. The results also indicated that chlorogenic acid contributes significantly to the observed effect. In addition, both fruit extracts exhibited antioxidant activity by scavenging DPPH and NO radicals with SC50 values of 153–352 µg/mL. Our study suggested that black chokeberry and bilberry fruits may be beneficial in cancer and other inflammation-related diseases.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms242115512