An off-the-shelf small extracellular vesicle nanomedicine for tumor targeting therapy

Small extracellular vesicles (sEVs) have shown excellent prospects as drug delivery systems for cancer therapy. However, the inherent non-targeting and short blood circulation characteristics severely restrict their practical applications as a delivery system. In addition, post-encapsulating drugs i...

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Bibliographic Details
Published in:Journal of controlled release 2023-12, Vol.364, p.672-686
Main Authors: Gong, Liang, Tian, Lu, Cui, Kaisa, Chen, Ying, Liu, Bingxin, Li, Dan, Feng, Yuyang, Yao, Surui, Yin, Yuan, Wu, Zhimeng, Huang, Zhaohui
Format: Article
Language:English
Subjects:
Antibodies
Biological Transport
Cell Line
Extracellular Vesicles
MicroRNAs
Nanomedicine
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Summary:Small extracellular vesicles (sEVs) have shown excellent prospects as drug delivery systems for cancer therapy. However, the inherent non-targeting and short blood circulation characteristics severely restrict their practical applications as a delivery system. In addition, post-encapsulating drugs into sEVs also remains challenging. Here, we constructed an engineered cell line that secreted multifunctional sEVs (termed NBsEV204) with 7D12 (an anti-EGFR nanobody) and hCD47 decorations on their surface, as well as high levels of miR-204-5p encapsulation. NBsEV204 exhibited extended blood circulation and improved macrophage-mediated phagocytosis of tumor cells by blocking CD47 signaling. Importantly, NBsEV204 specifically targeted EGFR tumor cells and showed robust tumor-suppressive effects both in vitro and in vivo. Overall, this study provides a convenient and feasible method to produce off-the-shelf anticancer sEV nanomedicine, which exhibits tremendous potential for clinical translation.
ISSN:0168-3659
1873-4995
1873-4995
DOI:10.1016/j.jconrel.2023.11.013