Gastric microbiota in patients with gastric MALT lymphoma according to Helicobacter pylori infection

• Published in: Clinics and research in hepatology and gastroenterology 2024-01, Vol.48 (1), p.102247, Article 102247
• Main Authors: Martin, Antoine, Jauvain, Marine, Bergsten, Emma, Demontant, Vanessa, Lehours, Philippe, Barau, Caroline, Levy, Michael, Rodriguez, Christophe, Sobhani, Iradj, Amiot, Aurelien
• Format: Article
• Language: English
• Subjects: Gastric Mucosa - microbiology; Helicobacter Infections - complications; Helicobacter Infections - microbiology; Helicobacter pylori - genetics; Humans; Lymphoma, B-Cell, Marginal Zone; Lymphoma, Non-Hodgkin; Microbiota; Retrospective Studies; RNA, Ribosomal, 16S - genetics; Stomach Neoplasms - genetics
• ISSN: 2210-7401; 2210-741X; 2210-741X
• DOI: 10.1016/j.clinre.2023.102247

Gastric Mucosa Associated Lymphoid Tissue lymphoma (GML) development is triggered by Helicobacter pylori (H. pylori) infection. Little is known about the impact of H. pylori infection on gastric microbiota. The gastric microbiota was retrospectively investigated using 16S rRNA gene sequencing in 32 patients with untreated GML (10 H. pylori-positive and 22 H. pylori-negative), 23 with remitted and 18 refractory GML and 35 controls. Differences in microbial diversity, bacterial composition and taxonomic repartition were assessed. There was no change in diversity and bacterial composition between GML and control patients taking into account H. pylori status. Differential taxa analysis identified specific changes associated with H. pylori-negative GML: the abundances of Actinobacillus, Lactobacillus and Chryseobacterium were increased while the abundances of Veillonella, Atopobium, Leptotrichia, Catonella, Filifactor and Escherichia_Shigella were increased in control patients. In patients with remitted GML, the genera Haemophilus and Moraxella were significantly more abundant than in refractory patients, while Atopobium and Actinomyces were significantly more abundant in refractory patients. Detailed analysis of the gastric microbiota revealed significant changes in the bacterial composition of the gastric mucosa in patients with GML that may have a role in gastric lymphomagenesis but not any new pathobionts.