Integrating Reliable Pt-S Bond-Mediated 3D DNA Nanomachine with Magnetic Separation in a Homogeneous Electrochemical Strategy for Exosomal MicroRNA Detection with Low Background and High Sensitivity

Precise and sensitive analysis of exosomal microRNA (miRNA) is of great importance for noninvasive early disease diagnosis, but it remains a great challenge to detect exosomal miRNA in human blood samples because of their small size, high sequence homology, and low abundance. Herein, we integrated r...

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Published in:Analytical chemistry (Washington) 2023-12, Vol.95 (48), p.17834-17842
Main Authors: Yang, Limin, Guo, Heng, Gao, Qian, Hou, Ting, Zhang, Jingang, Liu, Xiaojuan, Li, Feng
Format: Article
Language:English
Subjects:
Amplification
Biosensing Techniques - methods
Biosensors
Deoxyribonucleic acid
Diagnosis
DNA
DNA - analysis
Electrochemical Techniques - methods
Electrochemistry
Freezing
Homology
Humans
Limit of Detection
Liposomes
Magnetic Phenomena
Magnetic separation
MicroRNAs
MicroRNAs - analysis
miRNA
Physical Phenomena
Ribonucleic acid
RNA
Sensitivity analysis
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Summary:Precise and sensitive analysis of exosomal microRNA (miRNA) is of great importance for noninvasive early disease diagnosis, but it remains a great challenge to detect exosomal miRNA in human blood samples because of their small size, high sequence homology, and low abundance. Herein, we integrated reliable Pt-S bond-mediated three-dimensional (3D) DNA nanomachine and magnetic separation in a homogeneous electrochemical strategy for the detection of exosomal miRNA with low background and high sensitivity. The 3D DNA nanomachine was easily prepared via a facile and rapid freezing method, and it was capable of resisting the influence of biothiols, thus endowing it with high stability. Notably, the as-developed magnetic 3D DNA nanomachine not only enabled the detection system to have a low background but also coupled with liposome nanocarriers to synergistically amplify the current signal. Consequently, by ingeniously combining the low background and multiple signal-amplification strategies in homogeneous electrochemical biosensing, highly sensitive detection of exosomal miRNA was successfully achieved. More significantly, with good anti-interference ability, the as-proposed method could effectively discriminate plasma samples from cancer patients and healthy subjects, thus showing a high potential for application in the nondestructive early clinical diagnosis of disease.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.3c03914