Effect of crocin supplementation in the extender on the quality of chilled canine semen

The aim of the study was to evaluate the effects of crocin on canine sperm quality parameters during prolonged storage at 4 °C. Ejaculates from 10 dogs were diluted in a TRIS- egg yolk extender supplemented with 0 (control group), 0.5, 1, and 2 mM crocin and stored at 4 °C. Sperm membrane functional...

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Bibliographic Details
Published in:Animal reproduction science 2023-12, Vol.259, p.107374-107374, Article 107374
Main Authors: Calabria, Alfonso, Del Prete, Chiara, Ciarcia, Roberto, Longobardi, Valentina, Spada, Stefano, Alfano, Maria Teresa, De Felice, Daniela, Gasparrini, Bianca, Cocchia, Natascia
Format: Article
Language:English
Subjects:
Antioxidant
Dog
Semen storage
Spermatozoa
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Summary:The aim of the study was to evaluate the effects of crocin on canine sperm quality parameters during prolonged storage at 4 °C. Ejaculates from 10 dogs were diluted in a TRIS- egg yolk extender supplemented with 0 (control group), 0.5, 1, and 2 mM crocin and stored at 4 °C. Sperm membrane functional integrity, motility, and kinetics were assessed after 3 h, 24 h, 4 days and 7 days of storage. Based on the results, the more efficient concentration of crocin (0.5 mM) was chosen to evaluate sperm intracellular ROS levels, lipid peroxidation, and DNA fragmentation vs. the control. Semen with the addition of 0.5 mM crocin with respect to the control exhibited: i) increased (P 
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2023.107374