GSK-126 Attenuates Cell Apoptosis in Ischemic Brain Injury by Modulating the EZH2-H3K27me3-Bcl2l1 Axis

• Published in: Molecular neurobiology 2024-06, Vol.61 (6), p.3369-3383
• Main Authors: Zhou, Tai, Zhang, Lei, He, Li, Lan, Yan, Ding, Lei, Li, Li, Wang, Zhongcheng
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Apoptosis - drug effects; Bcl-2 protein; bcl-X Protein - metabolism; Biomedical and Life Sciences; Biomedicine; Brain Injuries - metabolism; Brain Injuries - pathology; Brain injury; Brain Ischemia - metabolism; Brain Ischemia - pathology; Cell Biology; Cerebral blood flow; Enhancer of Zeste Homolog 2 Protein - antagonists & inhibitors; Enhancer of Zeste Homolog 2 Protein - metabolism; Epigenetics; Excitotoxicity; Gene silencing; Histones; Histones - metabolism; Indoles; Infarction, Middle Cerebral Artery - metabolism; Infarction, Middle Cerebral Artery - pathology; Inflammation; Ischemia; Male; Methylation; Neurobiology; Neurology; Neurosciences; Oxidative stress; Oxidative Stress - drug effects; Pyridones; Stroke; Thiadiazoles - pharmacology
• ISSN: 0893-7648; 1559-1182
• DOI: 10.1007/s12035-023-03808-8

Whether epigenetic modifications participate in the cell apoptosis after ischemic stroke remains unclear. Histone 3 tri-methylation at lysine 27 (H3K27me3) is a histone modification that leads to gene silencing and is involved in the pathogenesis of ischemic stroke. Since the expression of many antiapoptotic genes is inhibited in the ischemic brains, here we aimed to offer an epigenetic solution to cell apoptosis after stroke by reversing H3K27me3 levels after ischemia. GSK-126, a specific inhibitor of enhancer of zeste homolog 2 (EZH2), significantly decreased H3K27me3 levels and inhibited middle cerebral artery occlusion (MCAO) induced and oxygen glucose deprivation (OGD) induced cell apoptosis. Moreover, GSK-126 attenuated the apoptosis caused by oxidative stress, excitotoxicity, and excessive inflammatory responses in vitro. The role of H3K27me3 in regulating of the expression of the antiapoptotic molecule B cell lymphoma-2 like 1 (Bcl2l1) explained the antiapoptotic effect of GSK-126. In conclusion, we found that GSK-126 could effectively protect brain cells from apoptosis after cerebral ischemia, and this role of GSK-126 is closely related to an axis that regulates Bcl2l1 expression, beginning with the regulation of EZH2-dependent H3K27me3 modification.