Solid-state fermentation production of L-lysine by Corynebacterium glutamicum (ATCC 13032) using agricultural by-products as substrate

To meet the growing demand for L-lysine, an essential amino acid with various applications, it is crucial to produce it on a large scale locally instead of relying solely on imports. This study aimed to evaluate the potential of using Corynebacterium glutamicum ATCC 13032 for L-lysine production fro...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2024-01, Vol.40 (1), p.20-20, Article 20
Main Authors: Egbune, Egoamaka O., Ezedom, Theresa, Odeghe, Otuke B., Orororo, Osuvwe C., Egbune, Olisemeke U., Ehwarieme, Ayobola D., Aganbi, Eferhire, Ebuloku, Chijindu S., Chukwuegbo, Alma O., Bogard, Ebiyeiferu, Ayomanor, Edesiri, Chisom, Patricia A., Edafetano, Fejiro L., Destiny, Albert, Alebe, Peace A., Aruwei, Toboke-keme, Anigboro, Akpovwehwee A., Tonukari, Nyerhovwo J.
Format: Article
Language:English
Subjects:
Amino acids
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Biotechnology
By products
Byproducts
Corynebacterium glutamicum
Corynebacterium glutamicum - metabolism
Elephant grass
Environmental Engineering/Biotechnology
Fermentation
Grasses
Groundnuts
Imports
Industrial applications
Life Sciences
Lysine
Microbiology
Pennisetum purpureum
Rice bran
Room temperature
Solid state
Solid state fermentation
Soybeans
Substrates
Xylanase
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Summary:To meet the growing demand for L-lysine, an essential amino acid with various applications, it is crucial to produce it on a large scale locally instead of relying solely on imports. This study aimed to evaluate the potential of using Corynebacterium glutamicum ATCC 13032 for L-lysine production from agricultural by-products such as palm kernel cake, soybean cake, groundnut cake, and rice bran. Solid-state fermentation was conducted at room temperature for 72 h, with the addition of elephant grass extract as a supplement. The results revealed that these agricultural by-products contain residual amounts of L-lysine. By employing solid-state fermentation with C. glutamicum (106 CFU/ml) in 100 g of various agricultural by-products, L-lysine production was achieved. Interestingly, the addition of elephant grass extract (1 g of elephant grass: 10 ml of water) further enhanced L-lysine production. Among the tested substrates, 100 g of groundnut cake moistened with 500 ml of elephant grass extract yielded the highest L-lysine concentration of 3.27 ± 0.02 (mg/gds). Furthermore, fermentation led to a substantial rise (p 
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-023-03822-x