High expression of integrin-binding sialoprotein (IBSP) is associated with poor prognosis of osteosarcoma

• Published in: Aging (Albany, NY.) NY.), 2023-11, Vol.16 (1), p.28
• Main Authors: Ma, Yihang, Chen, Bing, Zhang, Boyin, Zhang, Chao, Zhu, Qingsan, Wang, Xu, Liu, Zhengang, Liu, Haochuan
• Format: Article
• Language: English
• Subjects: Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; Bone Neoplasms - pathology; Humans; Integrin-Binding Sialoprotein; Neoplasm Recurrence, Local; Osteosarcoma - pathology; Prognosis
• ISSN: 1945-4589; 1945-4589
• DOI: 10.18632/aging.205235

Osteosarcoma is a malignant tumor, accounting for 20% of primary malignant bone tumors worldwide. However, the role of IBSP as a biomarker in osteosarcoma progression has not been studied yet. 85 cases of IBSP expression and clinical characteristics were obtained from TARGET database. Through the Kaplan-Meier curve, subgroup analysis, and univariate and multivariate Cox analysis, we further assessed the independent predictive capacity of IBSP expression for overall survival (OS) and relapse-free survival (RFS). The mRNA expression of IBSP was higher in osteosarcoma than normal tissue ( < 0.0001). IBSP expression grouped by vital status showed statistical differences ( = 0.042). The race ( = 0.0183), vital status ( = 0.0034), and sample type ( = 0.0020) showed significant differences. IBSP expression exhibited satisfied diagnostic ability for osteosarcoma. The univariate and multivariate analysis confirmed that IBSP expression was an independent risk factor for OS (HR = 3.425, 95% CI: 1.604-7.313, = 0.002) and RFS (HR = 3.377, 95% CI: 1.775-6.424, < 0.001) in osteosarcoma patients. High IBSP expression was significantly associated with poor OS and RFS ( < 0.0001). The higher IBSP expression was observed in osteosarcoma ( < 0.001), confirmed by the IHC staining. The CCK-8 and colony formation assay showed that IBSP knockdown inhibits cell proliferation while overexpression promotes cell proliferation ( < 0.05). High expression of IBSP was associated with poor OS and RFS. IBSP could serve as a potential biomarker for osteosarcoma, which could aid in early detection and disease monitoring.