Allyl methyl disulfide (AMDS) prevents N,N-dimethyl formamide-induced liver damage by suppressing oxidative stress and NLRP3 inflammasome activation

• Published in: Food and chemical toxicology 2023-12, Vol.182, p.114198-114198, Article 114198
• Main Authors: Li, Long-Xia, Wang, Lin, Wang, Shuo, Zhang, Xiu-Ning, Liu, Hong, Zhang, Yan-Jing, Wu, Chuan-Tao, Zhang, Cui-Li, Zeng, Tao
• Format: Article
• Language: English
• Subjects: Animals; Dimethylformamide - metabolism; Dimethylformamide - toxicity; Glutathione - metabolism; Humans; Inflammasomes - metabolism; Liver; Liver Diseases - metabolism; Mice; NF-kappa B - metabolism; NLR Family, Pyrin Domain-Containing 3 Protein - genetics; NLR Family, Pyrin Domain-Containing 3 Protein - metabolism; Oxidative Stress
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2023.114198

N,N-dimethylformamide (DMF), a widely consumed industrial solvent with persistent characteristics, can induce occupational liver damage and pose threats to the general population due to the enormous DMF-containing industrial efflux and emission from indoor facilities. This study was performed to explore the roles of allyl methyl disulfide (AMDS) in liver damage induced by DMF and the underlying mechanisms. AMDS was found to effectively suppress the elevation in the liver weight/body weight ratio and serum aminotransferase activities, and reduce the mortality of mice induced by DMF. In addition, AMDS abrogated DMF-elicited increases in malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) levels and decreases in glutathione (GSH) levels in mouse livers. The increase in macrophage number, mRNA expression of M1 macrophage biomarkers, and protein expression of key components in the NF-κB pathway and NLRP3 inflammasome induced by DMF exposure were all suppressed by AMDS in mouse livers. Furthermore, AMDS inhibited DMF-induced cell damage and NF-κB activation in cocultured AML12 hepatocytes and J774A.1 macrophages. However, AMDS per se did not significantly affect the protein level and activity of CYP2E1. Collectively, these results demonstrate that AMDS effectively ameliorates DMF-induced acute liver damage possibly by suppressing oxidative stress and inactivating the NF-κB pathway and NLRP3 inflammasome.