D27-like carotenoid isomerases: at the crossroads of strigolactone and abscisic acid biosynthesis

• Published in: Journal of experimental botany 2024-02, Vol.75 (4), p.1148-1158
• Main Authors: Tolnai, Zoltán, Sharma, Himani, Soós, Vilmos
• Format: Article
• Language: English
• Subjects: Abscisic Acid - metabolism; beta Carotene - metabolism; Carotenoids - metabolism; Heterocyclic Compounds, 3-Ring; Isomerases - metabolism; Lactones; Plant Proteins - metabolism; Xanthophylls
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/erad475

Abstract Strigolactones and abscisic acid (ABA) are apocarotenoid-derived plant hormones. Their biosynthesis starts with the conversion of trans-carotenes into cis forms, which serve as direct precursors. Iron-containing DWARF27 isomerases were shown to catalyse or contribute to the trans/cis conversions of these precursor molecules. D27 converts trans-β-carotene into 9-cis-β-carotene, which is the first committed step in strigolactone biosynthesis. Recent studies found that its paralogue, D27-LIKE1, also catalyses this conversion. A crucial step in ABA biosynthesis is the oxidative cleavage of 9-cis-violaxanthin and/or 9-cis-neoxanthin, which are formed from their trans isomers by unknown isomerases. Several lines of evidence point out that D27-like proteins directly or indirectly contribute to 9-cis-violaxanthin conversion, and eventually ABA biosynthesis. Apparently, the diversity of D27-like enzymatic activity is essential for the optimization of cis/trans ratios, and hence act to maintain apocarotenoid precursor pools. In this review, we discuss the functional divergence and redundancy of D27 paralogues and their potential direct contribution to ABA precursor biosynthesis. We provide updates on their gene expression regulation and alleged Fe–S cluster binding feature. Finally, we conclude that the functional divergence of these paralogues is not fully understood and we provide an outlook on potential directions in research. This review discusses the functional divergence and redundancy of D27-like carotenoid isomerases, their role in setting cis /trans carotenoid pools, and eventually their contribution to strigolactone and abscisic acid precursor biosynthesis.