Genomic profiling and characteristics of a C1 degrading heterotrophic fresh-water bacterium Paracoccus sp. strain DMF

• Published in: Archives of microbiology 2024-01, Vol.206 (1), p.6-6, Article 6
• Main Authors: Maurya, Shiwangi, Arya, Chetan Kumar, Parmar, Nidhi, Sathyanarayanan, Nitish, Joshi, Chaitanya G., Ramanathan, Gurunath
• Format: Article
• Language: English
• Subjects: Amines; Bacteria; Biochemistry; Biomedical and Life Sciences; Bioremediation; Biotechnology; Cell Biology; Degradation; Dimethylformamide; Domestic wastewater; Ecology; Gene sequencing; Genomes; Genomics; Gram-negative bacteria; Life Sciences; Methylotrophic bacteria; Microbial Ecology; Microbiology; Operons; Original Paper; Paracoccus; Paracoccus - genetics; Pathogenicity; Pathogens; Wastewater treatment; Wastewater treatment plants; Water
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-023-03729-z

Paracoccus species are metabolically versatile gram-negative, aerobic facultative methylotrophic bacteria showing enormous promise for environmental and bioremediation studies. Here we report, the complete genome analysis of Paracoccus sp . strain DMF ( P. DMF) that was isolated from a domestic wastewater treatment plant in Kanpur, India (26.4287 °N, 80.3891 °E) based on its ability to degrade a recalcitrant organic solvent N, N -dimethylformamide (DMF). The results reveal a genome size of 4,202,269 base pairs (bp) with a G + C content of 67.9%. The assembled genome comprises 4141 coding sequences (CDS), 46 RNA sequences, and 2 CRISPRs. Interestingly, catabolic operons related to the conventional marine-based methylated amines (MAs) degradation pathway were functionally annotated within the genome of an obligated aerobic heterotroph that is P . DMF. The genomic data-based characterization presented here for the novel heterotroph P. DMF aims to improve the understanding of the phenotypic gene products, enzymes, and pathways involved with greater emphasis on facultative methylotrophic motility-based latent pathogenicity.