Follistatin‐like 1 is a myokine regulating lipid mobilization during endurance exercise and recovery

• Published in: Obesity (Silver Spring, Md.) Md.), 2024-02, Vol.32 (2), p.352-362
• Main Authors: Nam, Ji Sun, Park, Su‐Jeong, Ahn, Chul Woo, Cho, Eun‐Suk, Kim, Hee‐Joo, Kim, YuSik
• Format: Article
• Language: English
• Subjects: Adipocytes; Biopsy; Body composition; Body fat; Cell culture; Cholesterol; Cyclic GMP - metabolism; Fitness training programs; Follistatin - metabolism; Follistatin-Related Proteins - genetics; Follistatin-Related Proteins - metabolism; Gene expression; Glucose; Glycerol; Glycerol - metabolism; Growth hormones; Humans; Insulin; Kinases; Lipid Mobilization; Lipids; Lipolysis - physiology; Lung cancer; Male; Metabolism; Musculoskeletal system; Myokines; Obesity; Peptides; Physiology; Proteins; Sterol Esterase - metabolism
• ISSN: 1930-7381; 1930-739X; 1930-739X
• DOI: 10.1002/oby.23949

Objective The aim of this study was to investigate the role of the follistatin‐like 1 (Fstl1) and disco‐interacting protein 2 homolog A (DIP2a) axis in relation to lipid metabolism during and after endurance exercise and to elucidate the mechanisms underlying the metabolic effects of Fstl1 on adipocytes, considering its regulation by exercise and muscle mass and its link to obesity. Methods Twenty‐nine sedentary males participated in endurance exercise, and blood samples were collected during and after the exercise. Body composition, Fstl1, glycerol, epinephrine, growth hormone, and atrial natriuretic peptide were measured. 3T3‐L1 adipocytes, with or without DIP2a knockdown, were treated with Fstl1 to assess glycerol release, cyclic AMP/cyclic GMP production, and hormone sensitive lipase phosphorylation. The association between DIP2a gene expression levels in human adipose tissues and exercise‐induced lipolysis was examined. Results Fstl1 levels significantly increased during endurance exercise and following recovery, correlating with lean body mass and lipolysis. In 3T3‐L1 adipocytes, Fstl1 increased glycerol release, cyclic GMP production, and hormone sensitive lipase activation, but these effects were attenuated by DIP2a knockdown. DIP2a gene expression in human adipose tissues correlated with serum glycerol concentrations during endurance exercise. Conclusions Fstl1 is a myokine facilitating lipid mobilization during and after endurance exercise through DIP2a‐mediated lipolytic effects in adipocytes.