Study on the effect of type III recombinant humanized collagen on human vascular endothelial cells

• Published in: Tissue engineering. Part C, Methods Methods, 2024-02, Vol.30 (ja), p.53-62
• Main Authors: Wang, Han, Xiao, Yonghao, Zhang, Yuanguo, Meng, Zhu, Zhao, Chenyu, Qiu, Fanshan, Li, Chongchong, Feng, Zengguo
• Format: Article
• Language: English
• ISSN: 1937-3384; 1937-3392
• DOI: 10.1089/ten.TEC.2023.0211

Methods: The effect of hCOLII on proliferation of EA.hy926 cells was detected by MTT assay, the effect of hCOLII on cell migration was investigated by scratch assay, the effect of hCOLIII on cell cycle and apoptosis was detected by flow cytometry, the ability of hCOLII to induce angiogenesis of EA.hy926 cells was evaluated by angiogenesis assay, and the effect of hCOLII on VEGF expression was detected by RT-PCR analysis. Results: The hCOLIII at concentrations of 0.5mg/ml, 0.25 mg/ml and 0.125 mg/ml all showed certain effects on the proliferation and migration of human vascular endothelial cells. It could also affect the cell cycle, increase the PI index and increase the expression level of VEGF in human vascular endothelial cells. In the meantime, hCOLIII at the concentration of 0.5mg/ml also showed a promoting effect on vessel formation. Conclusions: The hCOLIII has the potential to promote the endothelization process of blood vessels, mainly through affecting the proliferation, migration, and vascular-like structure of human endothelial cells. At the same time, hCOLIII can promote the expression of VEGF. This collagen demonstrated its potential as a raw material for cardiovascular implants.