Sir2‐mediated cytoplasmic deacetylation facilitates pathogenic fungi infection in host plants

Summary Lysine acetylation is an evolutionarily conserved and widespread post‐translational modification implicated in the regulation of multiple metabolic processes, but its function remains largely unknown in plant pathogenic fungi. A comprehensive analysis combined with proteomic, molecular and c...

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Bibliographic Details
Published in:The New phytologist 2024-02, Vol.241 (4), p.1732-1746
Main Authors: Zhang, Ning, Hu, Jicheng, Liu, Zhishan, Liang, Wenxing, Song, Limin
Format: Article
Language:English
Subjects:
Acetylation
Biogenesis
Chromatography, Liquid
deacetylase
Deacetylation
fungal infection
Fungi
Fungicides
Fusarium
Fusarium oxysporum
Host plants
Kinases
Lysine
lysine acetylation
Pathogenicity
Pathogens
Plant diseases
Plant Diseases - microbiology
Plants
post‐translational modification
Protein Processing, Post-Translational
Proteins
Proteomics
Ribosomes
Serine
Tandem Mass Spectrometry
Translation
Tyrosine
Virulence
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Summary:Summary Lysine acetylation is an evolutionarily conserved and widespread post‐translational modification implicated in the regulation of multiple metabolic processes, but its function remains largely unknown in plant pathogenic fungi. A comprehensive analysis combined with proteomic, molecular and cellular approaches was presented to explore the roles of cytoplasmic acetylation in Fusarium oxsysporum f.sp. lycopersici (Fol). The divergent cytoplasmic deacetylase FolSir2 was biochemically characterized, which is contributing to fungal virulence. Based on this, a total of 1752 acetylated sites in 897 proteins were identified in Fol via LC–MS/MS analysis. Further analyses of the quantitative acetylome revealed that 115 proteins representing two major pathways, translational and ribosome biogenesis, were hyperacetylated in the ∆Folsir2 strain. We experimentally examined the regulatory roles of FolSir2 on K271 deacetylation of FolGsk3, a serine/tyrosine kinase implicated in a variety of cellular functions, which was found to be crucial for the activation of FolGsk3 and thus modulated Fol pathogenicity. Cytoplasmic deacetylation by FolSir2 homologues has a similar function in Botrytis cinerea and likely other fungal pathogens. These findings reveal a conserved mechanism of silent information regulator 2‐mediated cytoplasmic deacetylation that is involved in plant‐fungal pathogenicity, providing a candidate target for designing broad‐spectrum fungicides to control plant diseases.
ISSN:0028-646X
1469-8137
DOI:10.1111/nph.19438