DNA Carrier-Assisted Molecular Ping-Pong in an Asymmetric Nanopore

Nanopore analysis relies on ensemble averaging of translocation signals obtained from numerous molecules, requiring a relatively high sample concentration and a long turnaround time from the sample to results. The recapture and subsequent re-reading of the same molecule is a promising alternative th...

Full description

Saved in:
Bibliographic Details
Published in:Nano letters 2023-12, Vol.23 (23), p.11145-11151
Main Authors: Zheng, Fei, Alawami, Mohammed, Zhu, Jinbo, Platnich, Casey M., Sha, Jingjie, Keyser, Ulrich F., Chen, Kaikai
Format: Article
Language:English
Subjects:
Diffusion
DNA - chemistry
Nanopores
Nanotechnology
Polymers
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nanopore analysis relies on ensemble averaging of translocation signals obtained from numerous molecules, requiring a relatively high sample concentration and a long turnaround time from the sample to results. The recapture and subsequent re-reading of the same molecule is a promising alternative that enriches the signal information from a single molecule. Here, we describe how an asymmetric nanopore improves molecular ping-pong by promoting the recapture of the molecule in the trans reservoir. We also demonstrate that the molecular recapture could be improved by linking the target molecule to a long DNA carrier to reduce the diffusion, thereby achieving over 100 recapture events. Using this ping-pong methodology, we demonstrate its use in accurately resolving nanostructure motifs along a DNA scaffold through repeated detection. Our method offers novel insights into the control of DNA polymer dynamics within nanopore confinement and opens avenues for the development of a high-fidelity DNA detection platform.
ISSN:1530-6984
1530-6992
DOI:10.1021/acs.nanolett.3c03605