Inhibition of PQS signaling by the Pf bacteriophage protein PfsE enhances viral replication in Pseudomonas aeruginosa

• Published in: Molecular microbiology 2024-01, Vol.121 (1), p.116-128
• Main Authors: Schwartzkopf, Caleb M., Taylor, Véronique L., Groleau, Marie‐Christine, Faith, Dominick R., Schmidt, Amelia K., Lamma, Tyrza L., Brooks, Diane M., Déziel, Eric, Maxwell, Karen L., Secor, Patrick R.
• Format: Article
• Language: English
• Subjects: Bacteria; Bacterial Proteins - metabolism; Bacteriophages - metabolism; Biosynthesis; Cell density; Chromosomes; filamentous Pf bacteriophage; Opportunist infection; Phages; phage‐bacteria interactions; Pili; PQS quorum sensing; Prophages; Proteins; Pseudomonas aeruginosa; Pseudomonas aeruginosa - genetics; pyocyanin virulence factor; Quinolones; Quorum sensing; Quorum Sensing - genetics; quorum sensing inhibitor; Replication; Signal Transduction; Signalling systems; Virions; Virus Replication
• ISSN: 0950-382X; 1365-2958
• DOI: 10.1111/mmi.15202

Quorum sensing, a bacterial signaling system that coordinates group behaviors as a function of cell density, plays an important role in regulating viral (phage) defense mechanisms in bacteria. The opportunistic pathogen Pseudomonas aeruginosa is a model system for the study of quorum sensing. P. aeruginosa is also frequently infected by Pf prophages that integrate into the host chromosome. Upon induction, Pf phages suppress host quorum sensing systems; however, the physiological relevance and mechanism of suppression are unknown. Here, we identify the Pf phage protein PfsE as an inhibitor of Pseudomonas Quinolone Signal (PQS) quorum sensing. PfsE binds to the host protein PqsA, which is essential for the biosynthesis of the PQS signaling molecule. Inhibition of PqsA increases the replication efficiency of Pf virions when infecting a new host and when the Pf prophage switches from lysogenic replication to active virion replication. In addition to inhibiting PQS signaling, our prior work demonstrates that PfsE also binds to PilC and inhibits type IV pili extension, protecting P. aeruginosa from infection by type IV pili‐dependent phages. Overall, this work suggests that the simultaneous inhibition of PQS signaling and type IV pili by PfsE may be a viral strategy to suppress host defenses to promote Pf replication while at the same time protecting the susceptible host from competing phages. Quorum sensing regulates phage defense in Pseudomonas aeruginosa. The Pf phage protein PfsE inhibits PQS‐mediated quorum sensing by binding to the host enzyme PqsA, while also protecting against type IV pili‐dependent phage infection. This dual inhibition strategy promotes Pf replication and safeguards the host from competing phages.