Survival, growth and digestive functions after exposure to nanodiamonds - Transgenerational effects beyond contact time in house cricket strains

• Published in: Chemosphere (Oxford) 2024-02, Vol.349, p.140809-140809, Article 140809
• Main Authors: Augustyniak, Maria, Ajay, Amrendra K., Kędziorski, Andrzej, Tarnawska, Monika, Rost-Roszkowska, Magdalena, Flasz, Barbara, Babczyńska, Agnieszka, Mazur, Beata, Rozpędek, Katarzyna, Alian, Reyhaneh Seyed, Skowronek, Magdalena, Świerczek, Ewa, Wiśniewska, Klaudia, Ziętara, Patrycja
• Format: Article
• Language: English
• Subjects: Food budget; Growth curves; Gut enzymes fingerprints; Kaplan-meier survival analysis; Midgut epithelium histology
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2023.140809

The long-term exposure effects of nanodiamonds (NDs), spanning an organism's entire lifespan and continuing for subsequent generation, remain understudied. Most research has focused on evaluating their biological impacts on cell lines and selected organisms, typically over short exposure durations lasting hours or days. The study aimed to assess growth, mortality, and digestive functions in wild (H) and long-lived (D) strains of Acheta domesticus (Insecta: Orthoptera) after two-generational exposure to NDs in concentrations of 0.2 or 2 mg kg−1 of food, followed by their elimination in the third generation. NDs induced subtle stimulating effect that depended on the strain and generation. In the first generation, more such responses occurred in the H than in the D strain. In the first generation of H strain insects, contact with NDs increased survival, stimulated the growth of young larvae, and the activity of most digestive enzymes in mature adults. The same doses and exposure time did not cause similar effects in the D strain. In the first generation of D strain insects, survival and growth were unaffected by NDs, whereas, in the second generation, significant stimulation of those parameters was visible. Selection towards longevity appears to support higher resistance of the insects to exposure to additional stressor, at least in the first generation. The cessation of ND exposure in the third generation caused potentially harmful changes, which included, e.g., decreased survival probability in H strain insects, slowed growth of both strains, as well as changes in heterochromatin density and distribution in nuclei of the gut cells in both strains. Such a reaction may suggest the involvement of epigenetic inheritance mechanisms, which may become inadequate after the stress factor is removed. [Display omitted] •Nanodiamonds (NDs) can induce strain- and generation-dependent disorders.•The specific population life history can modify NDs-induced effects.•Selection for longevity improves tolerance to oxidative stress-mediated factors.•Cessation of ND exposure in the 3rd generation (G3) can cause harmful effects.•The G3 individuals showed changes in the distribution and density of heterochromatin.