CRMP5 participates in oocyte meiosis by regulating spastin to correct microtubule-kinetochore misconnection

Our previous studies have suggested that spastin, which aggregates on spindle microtubules in oocytes, may promote the assembly of mouse oocyte spindles by cutting microtubules. This action may be related to CRMP5, as knocking down CRMP5 results in reduced spindle microtubule density and maturation...

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Published in:Zygote (Cambridge) 2024-02, Vol.32 (1), p.21-27
Main Authors: Jin, Zhen, Zhang, Zhi-Cai, Xiao, Chen-Yu, Li, Mei-Qi, Li, Qian-Ru, Gao, Lei-Lei
Format: Article
Language:English
Subjects:
Abnormalities
Animals
Antibodies
Cell division
Chromosomes
Defects
Errors
Gametocytes
In vitro fertilization
Kinetochores - metabolism
Maturation
Meiosis
Mice
Microtubules
Microtubules - metabolism
Oocytes
Oocytes - physiology
Ovaries
Poles
Polymerization
Proteins
Regulatory mechanisms (biology)
RNA polymerase
Spastin - genetics
Spastin - metabolism
Spindle Apparatus - physiology
Spindles
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Summary:Our previous studies have suggested that spastin, which aggregates on spindle microtubules in oocytes, may promote the assembly of mouse oocyte spindles by cutting microtubules. This action may be related to CRMP5, as knocking down CRMP5 results in reduced spindle microtubule density and maturation defects in oocytes. In this study, we found that, after knocking down CRMP5 in oocytes, spastin distribution shifted from the spindle to the spindle poles and errors in microtubule-kinetochore attachment appeared in oocyte spindles. However, CRMP5 did not interact with the other two microtubule-severing proteins, katanin-like-1 (KATNAL1) and fidgetin-like-1 (FIGNL1), which aggregate at the spindle poles. We speculate that, in oocytes, due to the reduction of spastin distribution on chromosomes after knocking down CRMP5, microtubule-kinetochore errors cannot be corrected through severing, resulting in meiotic division abnormalities and maturation defects in oocytes. This finding provides new insights into the regulatory mechanisms of spastin in oocytes and important opportunities for the study of meiotic division mechanisms.
ISSN:0967-1994
1469-8730
DOI:10.1017/S0967199423000564