Wet chemical synthesis of TGA capped Ag2S nanoparticles and their use for fluorescence imaging and temperature sensing in living cells

In this work, we describe a simple wet chemical route for preparing silver sulfide nanoparticles (Ag2S) encapsulated with thioglycolic acid (TGA). By using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), energy dispersive X-ray (EDS) microanalysis, transmission electron micr...

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Bibliographic Details
Published in:RSC advances 2023-11, Vol.13 (50), p.35065-35077
Main Authors: Ibrahim, Mayssa, Camarero, P, Ming, Liyan, Haouari, Mohamed, Amamou, Noura, Haro-González, P, Fredj Hassen
Format: Article
Language:English
Subjects:
Biocompatibility
Cells (biology)
Chemical synthesis
Fluorescent indicators
Fourier transforms
Infrared spectroscopy
Nanoparticles
Photoluminescence
Photon correlation spectroscopy
Silver
Spectrum analysis
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Summary:In this work, we describe a simple wet chemical route for preparing silver sulfide nanoparticles (Ag2S) encapsulated with thioglycolic acid (TGA). By using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), energy dispersive X-ray (EDS) microanalysis, transmission electron microscopy (TEM), and dynamic light scattering (DLS), we have found that these nanoparticles were enrobed by TGA molecules and they have an Ag/S ratio nearly equal to 2.2 and a nearly spherical shape with two average size populations. Photoluminescence (PL) spectroscopy has shown that these nanoparticles are highly luminescent, photostable and photobleaching resistant and they emit in the first biologic window with a band peaking in the NIR region at 915 nm. We have demonstrated through a 3-(4,5-dimethyl-thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay protocol and using U-87 MG human living cells that these nanoparticles are biocompatible with a viability ratio higher than 80% for a concentration equal to 100 μg mL−1. By investigating the effect of pH, ionic strength and thermal quenching on the PL emission, we have shown that these nanoparticles provide a convenient stable tool to measure temperature in the biological range with a relative thermal sensitivity higher than 5% per °C and they may be used as suitable fluorescent probes for living cell imaging and intracellular temperature mapping.
ISSN:2046-2069
DOI:10.1039/d3ra06705j