HERPUD1 governs tumor cell mitochondrial function via inositol 1,4,5-trisphosphate receptor-mediated calcium signaling

• Published in: Free radical biology & medicine 2024-02, Vol.211, p.24-34
• Main Authors: Paredes, Felipe, Navarro-Marquez, Mario, Quiroga, Clara, Jiménez-Gallegos, Danica, Yeligar, Samantha M., Parra, Valentina, Müller, Marioly, Chiong, Mario, Quest, Andrew F.G., San Martin, Alejandra, Lavandero, Sergio
• Format: Article
• Language: English
• Subjects: Calcium; Calcium - metabolism; Calcium Signaling - physiology; HERPUD1; Humans; Inositol - metabolism; Inositol 1,4,5-Trisphosphate Receptors - genetics; Inositol 1,4,5-Trisphosphate Receptors - metabolism; IP3 receptor; Mitochondria; Mitochondria - genetics; Mitochondria - metabolism; Neoplasms - genetics; Neoplasms - metabolism; Oxidative stress; Transcription Factors - metabolism; Tumoral cell
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/j.freeradbiomed.2023.11.022

The intricate relationship between calcium (Ca2+) homeostasis and mitochondrial function is crucial for cellular metabolic adaptation in tumor cells. Ca2+-initiated signaling maintains mitochondrial respiratory capacity and ATP synthesis, influencing critical cellular processes in cancer development. Previous studies by our group have shown that the homocysteine-inducible ER Protein with Ubiquitin-Like Domain 1 (HERPUD1) regulates inositol 1,4,5-trisphosphate receptor (ITPR3) levels and intracellular Ca2+ signals in tumor cells. This study explores the role of HERPUD1 in regulating mitochondrial function and tumor cell migration by controlling ITPR3-dependent Ca2+ signals. We found HERPUD1 levels correlated with mitochondrial function in tumor cells, with HERPUD1 deficiency leading to enhanced mitochondrial activity. HERPUD1 knockdown increased intracellular Ca2+ release and mitochondrial Ca2+ influx, which was prevented using the ITPR3 antagonist xestospongin C or the Ca2+ chelator BAPTA-AM. Furthermore, HERPUD1 expression reduced tumor cell migration by controlling ITPR3-mediated Ca2+ signals. HERPUD1-deficient cells exhibited increased migratory capacity, which was attenuated by treatment with xestospongin C or BAPTA-AM. Additionally, HERPUD1 deficiency led to reactive oxygen species-dependent activation of paxillin and FAK proteins, which are associated with enhanced cell migration. Our findings highlight the pivotal role of HERPUD1 in regulating mitochondrial function and cell migration by controlling intracellular Ca2+ signals mediated by ITPR3. Understanding the interplay between HERPUD1 and mitochondrial Ca2+ regulation provides insights into potential therapeutic targets for cancer treatment and other pathologies involving altered energy metabolism. [Display omitted] •HERPUD1 expression is regulated and correlates with mitochondrial function in tumor cells.•HERPUD1 deficiency increases mitochondrial calcium influx via the inositol trisphosphate receptor.•HERPUD1 modulates tumor cell migration through inositol trisphosphate receptor-mediated calcium signals.•HERPUD1 deficiency led to ROS-dependent paxillin and FAK activation and cell migration.•HERPUD1 expression correlates with cancer survival rates in humas.