Development of a fumonisin-sensitive Saccharomyces cerevisiae indicator strain and utilization for activity testing of candidate detoxification genes

Fumonisins can cause diseases in animals and humans consuming -contaminated food or feed. The search for microbes capable of fumonisin degradation, or for enzymes that can detoxify fumonisins, currently relies primarily on chemical detection methods. Our constructed fumonisin B1-sensitive yeast stra...

Full description

Saved in:
Bibliographic Details
Published in:Applied and environmental microbiology 2023-12, Vol.89 (12), p.e0121123-e0121123
Main Authors: Krska, Tamara, Twaruschek, Krisztian, Valente, Nina, Mitterbauer, Rudolf, Moll, Dieter, Wiesenberger, Gerlinde, Berthiller, Franz, Adam, Gerhard
Format: Article
Language:English
Subjects:
Animal Feed
Animals
Apoptosis
ATP-binding protein
Baking yeast
Biosynthesis
Casein
Casein kinase II
Cell death
Ceramide
Coding
Detoxification
Efflux
Environmental Microbiology
Eukaryotes
Fumonisin B1
Fumonisins - metabolism
Fumonisins - toxicity
Fusarium - genetics
Fusarium - metabolism
Genes
Genetics and Molecular Biology
Humans
Kinases
Mycotoxins
Peptones
Phosphorylation
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Sphinganine
Toxicity
Uracil
Yeast
Yeasts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fumonisins can cause diseases in animals and humans consuming -contaminated food or feed. The search for microbes capable of fumonisin degradation, or for enzymes that can detoxify fumonisins, currently relies primarily on chemical detection methods. Our constructed fumonisin B1-sensitive yeast strain can be used to phenotypically detect detoxification activity and should be useful in screening for novel fumonisin resistance genes and to elucidate fumonisin metabolism and resistance mechanisms in fungi and plants, and thereby, in the long term, help to mitigate the threat of fumonisins in feed and food.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.01211-23