Stem cells derived from human exfoliated deciduous teeth-based media in a rat root resorption model

• Published in: Archives of oral biology 2024-02, Vol.158, p.105854-105854, Article 105854
• Main Authors: Odo, Ayaka, Kunimatsu, Ryo, Abe, Takaharu, Sakata, Shuzo, Nakatani, Ayaka, Rikitake, Kodai, Koizumi, Yuma, Tanabe, Izumi, Okimura, Naonobu, Yoshimi, Yuki, Tanimoto, Kotaro
• Format: Article
• Language: English
• Subjects: Animals; Deciduous teeth; Human; Humans; Interleukin-17 - metabolism; Interleukin-1beta - metabolism; Interleukin-6 - metabolism; Male; Osteoclasts; Osteoprotegerin - metabolism; RANK Ligand - metabolism; Rats; Rats, Sprague-Dawley; Root resorption; Root Resorption - metabolism; Stem cells; Stem Cells - metabolism; Tooth Movement Techniques; Tooth, Deciduous; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/j.archoralbio.2023.105854

Root resorption may occur during orthodontic treatment. Herein, we investigated the effect of a culture supernatant of stem cells derived from human exfoliated deciduous teeth on root resorption. Twelve 8-week-old male Sprague–Dawley rats were used, and their maxillary first molars were pulled with excessive orthodontic force to induce root resorption. On days 1 and 7 after traction initiation, stem cells derived from human exfoliated deciduous teeth and alpha minimum essential medium (control group) were administered. After 14 days, the maxillary bone was evaluated for tooth movement. The expression of osteoprotegerin, receptor activator of nuclear factor κB ligand, tumor necrosis factor α, interleukin 1β, interleukin 6, and interleukin 17 was evaluated on the compression side and tension side. No significant difference in tooth movement was observed between the two groups. Root resorption decreased in the group administered the culture supernatant compared with in the control. Immunohistochemical staining revealed increased osteoprotegerin expression and decreased receptor activators for nuclear factor κB ligand, tumor necrosis factor α, interleukin 1β, interleukin 6, and interleukin 17 on the compression side and tension side. Administration of stem cells derived from human exfoliated deciduous teeth affected the expression of osteoprotegerin, receptor activator of nuclear factor κB ligand, tumor necrosis factor α, interleukin 1β, interleukin 6 and interleukin 17; hence, these stem cells may inhibit root resorption by regulating their expression. •SHED-CM administration in rats inhibited root resorption.•SHED-CM administration did not affect tooth movement distance.•Osteoprotegerin expression was increased in the culture supernatant group.•NF-κB ligand, TNF-α, interleukin-1β, − 6, and − 17 expression was decreased.