Optogenetic action potentials and intrinsic pacemaker interplay in retrogradely identified midbrain dopamine neurons

• Published in: The European journal of neuroscience 2024-03, Vol.59 (6), p.1311-1331
• Main Authors: Hammer, Niklas, Vogel, Pascal, Lee, Sanghun, Roeper, Jochen
• Format: Article
• Language: English
• Subjects: Axon guidance; Caudate-putamen; Dopamine; Dopamine transporter; Mesencephalon; Motivation; Neostriatum; Neurons; optotagging; Pacemakers; retrograde viral tracing; sparse labelling; Substantia nigra
• ISSN: 0953-816X; 1460-9568
• DOI: 10.1111/ejn.16208

Dissecting the diversity of midbrain dopamine (DA) neurons by optotagging is a promising addition to better identify their functional properties and contribution to motivated behavior. Retrograde molecular targeting of DA neurons with specific axonal projection allows further refinement of this approach. Here, we focus on adult mouse DA neurons in the substantia nigra pars compacta (SNc) projecting to dorsal striatum (DS) by demonstrating the selectivity of a floxed AAV9‐based retrograde channelrhodopsin‐eYFP (ChR‐eYFP) labeling approach in DAT‐cre mice. Furthermore, we show the utility of a sparse labeling version for anatomical single‐cell reconstruction and demonstrate that ChR‐eYFR expressing DA neurons retain intrinsic functional properties indistinguishable from conventionally retrogradely red‐beads‐labeled neurons. We systematically explore the properties of optogenetically evoked action potentials (oAPs) and their interaction with intrinsic pacemaking in this defined subpopulation of DA neurons. We found that the shape of the oAP and its first derivative, as a proxy for extracellularly recorded APs, is highly distinct from spontaneous APs (sAPs) of the same neurons and systematically varies across the pacemaker duty cycle. The timing of the oAP also affects the backbone oscillator of the intrinsic pacemaker by introducing transient “compensatory pauses”. Characterizing this systematic interplay between oAPs and sAPs in defined DA neurons will also facilitate a refinement of DA neuron optotagging in vivo. Refinement of retrograde viral labelling of midbrain dopamine neurons (left column). The waveform of optogenetically triggered action potentials deviates significant from spontaneous action potentials (right column).