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Assessment of antiarthritic potential of Asparagus dumosus using formaldehyde and CFA-induced arthritic models in rats via modulation of oxidative stress biomarkers and mRNA expression of IL-1b, IL-6, RANKL, OPG, TNF-α and COX-2
Medicinal plants play a pivotal role in the prevention of chronic non-communicable diseases including arthritis. Despite the traditional use of Asparagus dumosus in arthritis, it has not been studied yet for its effectiveness in arthritis. This study was aimed to explore the antiarthritic potential...
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| Published in: | Inflammopharmacology 2024-02, Vol.32 (1), p.825-847 |
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| creator | Nazar, Nimra Mehmood, Malik Hassan Siddique, Rida Faisal, Muhammad Naeem |
| description | Medicinal plants play a pivotal role in the prevention of chronic non-communicable diseases including arthritis. Despite the traditional use of
Asparagus dumosus
in arthritis, it has not been studied yet for its effectiveness in arthritis. This study was aimed to explore the antiarthritic potential of
A. dumosus
in formaldehyde and complete Freund’s adjuvant (CFA)-induced arthritic rats. Body weight, arthritic index, hepatic oxidative stress, hematological, biochemical and inflammatory markers were assessed using ELISA, whilst qRT-PCR studies were carried out for the mRNA expression of IL-1b, IL-6, RANKL, OPG, TNF-α and COX-2 genes. GCMS and HPLC analysis were performed to identify the secondary metabolites of
A. dumosus
. From day 8 to 28 post-administration of formaldehyde and CFA, oral administration of
A. dumosus
(600, 300 and 150 mg/kg) showed a noteworthy improvement (
p
|
| doi_str_mv | 10.1007/s10787-023-01391-x |
| format | article |
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Asparagus dumosus
in arthritis, it has not been studied yet for its effectiveness in arthritis. This study was aimed to explore the antiarthritic potential of
A. dumosus
in formaldehyde and complete Freund’s adjuvant (CFA)-induced arthritic rats. Body weight, arthritic index, hepatic oxidative stress, hematological, biochemical and inflammatory markers were assessed using ELISA, whilst qRT-PCR studies were carried out for the mRNA expression of IL-1b, IL-6, RANKL, OPG, TNF-α and COX-2 genes. GCMS and HPLC analysis were performed to identify the secondary metabolites of
A. dumosus
. From day 8 to 28 post-administration of formaldehyde and CFA, oral administration of
A. dumosus
(600, 300 and 150 mg/kg) showed a noteworthy improvement (
p
< 0.001) in the body weights, immune organ weights, serum levels of rheumatoid (RA) factor, C-reactive protein, TNF-α and IL-6 levels in arthritic rats similar to the effect of piroxicam and methotrexate. Subsequently, the administration of
A. dumosus
to formaldehyde and CFA-challenged rats, caused a marked decrease (
p
< 0.001) in the mRNA expression of IL-1b, IL-6, OPG, RANKL, TNF-α and COX-2 genes in treated rats. Likewise, when assessed for antioxidant potential,
A. dumosus
produced a pronounced (
p
< 0.001) reduction in malondialdehyde (MDA) levels and hydrogen peroxide (H
2
O
2
) production, whilst a dose-dependent (
p
< 0.001) increase in catalase (CAT) and superoxide dismutase (SOD) activities was recorded. GCMS profiling of
A. dumosus
presented benzaldehyde, 3-hydroxy-4-methoxy-, 1-decanol and undecane as plant compositions, whereas HPLC fingerprinting displayed quercetin, benzaldehyde, 3-hydroxy-4-methoxy-, gallic acid and cinnamic acid as plants constituents. These results depict that
A. dumosus
possesses anti-arthritic effect mediated possibly through attenuation of arthritic indices, chronic inflammatory and oxidative stress biomarkers along with down-regulation in the mRNA expression of arthritic candid genes.</description><identifier>ISSN: 0925-4692</identifier><identifier>EISSN: 1568-5608</identifier><identifier>DOI: 10.1007/s10787-023-01391-x</identifier><identifier>PMID: 38057565</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Allergology ; Animals ; Arthritis ; Benzaldehydes ; Biomarkers ; Biomedical and Life Sciences ; Biomedicine ; Cyclooxygenase 2 - genetics ; Dermatology ; Formaldehyde ; Freund's Adjuvant ; Gastroenterology ; Hydrogen Peroxide ; Immunology ; Interleukin-6 ; Original Article ; Oxidative Stress ; Pharmacology/Toxicology ; Rats ; Rheumatology ; RNA, Messenger - genetics ; Tumor Necrosis Factor-alpha - genetics</subject><ispartof>Inflammopharmacology, 2024-02, Vol.32 (1), p.825-847</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Switzerland AG 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-c88ec9a8b07a7ee9c60f3eefa1e4baa9519f315e951ae832c45d977016fc9eab3</citedby><cites>FETCH-LOGICAL-c347t-c88ec9a8b07a7ee9c60f3eefa1e4baa9519f315e951ae832c45d977016fc9eab3</cites><orcidid>0000-0002-9959-1458</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38057565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nazar, Nimra</creatorcontrib><creatorcontrib>Mehmood, Malik Hassan</creatorcontrib><creatorcontrib>Siddique, Rida</creatorcontrib><creatorcontrib>Faisal, Muhammad Naeem</creatorcontrib><title>Assessment of antiarthritic potential of Asparagus dumosus using formaldehyde and CFA-induced arthritic models in rats via modulation of oxidative stress biomarkers and mRNA expression of IL-1b, IL-6, RANKL, OPG, TNF-α and COX-2</title><title>Inflammopharmacology</title><addtitle>Inflammopharmacol</addtitle><addtitle>Inflammopharmacology</addtitle><description>Medicinal plants play a pivotal role in the prevention of chronic non-communicable diseases including arthritis. Despite the traditional use of
Asparagus dumosus
in arthritis, it has not been studied yet for its effectiveness in arthritis. This study was aimed to explore the antiarthritic potential of
A. dumosus
in formaldehyde and complete Freund’s adjuvant (CFA)-induced arthritic rats. Body weight, arthritic index, hepatic oxidative stress, hematological, biochemical and inflammatory markers were assessed using ELISA, whilst qRT-PCR studies were carried out for the mRNA expression of IL-1b, IL-6, RANKL, OPG, TNF-α and COX-2 genes. GCMS and HPLC analysis were performed to identify the secondary metabolites of
A. dumosus
. From day 8 to 28 post-administration of formaldehyde and CFA, oral administration of
A. dumosus
(600, 300 and 150 mg/kg) showed a noteworthy improvement (
p
< 0.001) in the body weights, immune organ weights, serum levels of rheumatoid (RA) factor, C-reactive protein, TNF-α and IL-6 levels in arthritic rats similar to the effect of piroxicam and methotrexate. Subsequently, the administration of
A. dumosus
to formaldehyde and CFA-challenged rats, caused a marked decrease (
p
< 0.001) in the mRNA expression of IL-1b, IL-6, OPG, RANKL, TNF-α and COX-2 genes in treated rats. Likewise, when assessed for antioxidant potential,
A. dumosus
produced a pronounced (
p
< 0.001) reduction in malondialdehyde (MDA) levels and hydrogen peroxide (H
2
O
2
) production, whilst a dose-dependent (
p
< 0.001) increase in catalase (CAT) and superoxide dismutase (SOD) activities was recorded. GCMS profiling of
A. dumosus
presented benzaldehyde, 3-hydroxy-4-methoxy-, 1-decanol and undecane as plant compositions, whereas HPLC fingerprinting displayed quercetin, benzaldehyde, 3-hydroxy-4-methoxy-, gallic acid and cinnamic acid as plants constituents. These results depict that
A. dumosus
possesses anti-arthritic effect mediated possibly through attenuation of arthritic indices, chronic inflammatory and oxidative stress biomarkers along with down-regulation in the mRNA expression of arthritic candid genes.</description><subject>Allergology</subject><subject>Animals</subject><subject>Arthritis</subject><subject>Benzaldehydes</subject><subject>Biomarkers</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cyclooxygenase 2 - genetics</subject><subject>Dermatology</subject><subject>Formaldehyde</subject><subject>Freund's Adjuvant</subject><subject>Gastroenterology</subject><subject>Hydrogen Peroxide</subject><subject>Immunology</subject><subject>Interleukin-6</subject><subject>Original Article</subject><subject>Oxidative Stress</subject><subject>Pharmacology/Toxicology</subject><subject>Rats</subject><subject>Rheumatology</subject><subject>RNA, Messenger - genetics</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><issn>0925-4692</issn><issn>1568-5608</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9UU1u1DAUjhCIDoULsEBeshiDHU_ieBmNmFIxmkFVkdhFTvIydUni4OdU02Nxkd6i98BpBtix-uz3_eg9fVH0lrMPnDH5ETmTmaQsFpRxoTg9PosWPEkzmqQsex4tmIoTukpVfBa9QrxljKUyVS-jM5GxRCZpsogec0RA7KD3xDZE995o52-c8aYig_UwDdqJynHQTh9GJPXYWQw4oukPpLGu020NN_c1BH9N1pucmr4eK6jJv6zO1tAiMT1x2iO5M3oaja32xvZTvj2aOnzugKB3YSVSGttp9wMcPsV2V7ucwHGYuJPlckt5uZwgXZKrfPdluyT7rxdLcr3b0Idf8zb77zR-Hb1odIvw5oTn0bfNp-v1Z7rdX1yu8y2txEp6WmUZVEpnJZNaAqgqZY0AaDSHVam1SrhqBE8gPDRkIq5WSa2kZDxtKgW6FOfR-zl3cPbnCOiLzmAFbat7sCMWcaaUkHEsZJDGs7RyFtFBUwzOhHvvC86Kqd5irrcI9RZP9RbHYHp3yh_LDuq_lj99BoGYBRio_gCuuLWj68PN_4v9DdpotL8</recordid><startdate>20240201</startdate><enddate>20240201</enddate><creator>Nazar, Nimra</creator><creator>Mehmood, Malik Hassan</creator><creator>Siddique, Rida</creator><creator>Faisal, Muhammad Naeem</creator><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9959-1458</orcidid></search><sort><creationdate>20240201</creationdate><title>Assessment of antiarthritic potential of Asparagus dumosus using formaldehyde and CFA-induced arthritic models in rats via modulation of oxidative stress biomarkers and mRNA expression of IL-1b, IL-6, RANKL, OPG, TNF-α and COX-2</title><author>Nazar, Nimra ; Mehmood, Malik Hassan ; Siddique, Rida ; Faisal, Muhammad Naeem</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-c88ec9a8b07a7ee9c60f3eefa1e4baa9519f315e951ae832c45d977016fc9eab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Allergology</topic><topic>Animals</topic><topic>Arthritis</topic><topic>Benzaldehydes</topic><topic>Biomarkers</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cyclooxygenase 2 - genetics</topic><topic>Dermatology</topic><topic>Formaldehyde</topic><topic>Freund's Adjuvant</topic><topic>Gastroenterology</topic><topic>Hydrogen Peroxide</topic><topic>Immunology</topic><topic>Interleukin-6</topic><topic>Original Article</topic><topic>Oxidative Stress</topic><topic>Pharmacology/Toxicology</topic><topic>Rats</topic><topic>Rheumatology</topic><topic>RNA, Messenger - genetics</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nazar, Nimra</creatorcontrib><creatorcontrib>Mehmood, Malik Hassan</creatorcontrib><creatorcontrib>Siddique, Rida</creatorcontrib><creatorcontrib>Faisal, Muhammad Naeem</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Inflammopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nazar, Nimra</au><au>Mehmood, Malik Hassan</au><au>Siddique, Rida</au><au>Faisal, Muhammad Naeem</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of antiarthritic potential of Asparagus dumosus using formaldehyde and CFA-induced arthritic models in rats via modulation of oxidative stress biomarkers and mRNA expression of IL-1b, IL-6, RANKL, OPG, TNF-α and COX-2</atitle><jtitle>Inflammopharmacology</jtitle><stitle>Inflammopharmacol</stitle><addtitle>Inflammopharmacology</addtitle><date>2024-02-01</date><risdate>2024</risdate><volume>32</volume><issue>1</issue><spage>825</spage><epage>847</epage><pages>825-847</pages><issn>0925-4692</issn><eissn>1568-5608</eissn><abstract>Medicinal plants play a pivotal role in the prevention of chronic non-communicable diseases including arthritis. Despite the traditional use of
Asparagus dumosus
in arthritis, it has not been studied yet for its effectiveness in arthritis. This study was aimed to explore the antiarthritic potential of
A. dumosus
in formaldehyde and complete Freund’s adjuvant (CFA)-induced arthritic rats. Body weight, arthritic index, hepatic oxidative stress, hematological, biochemical and inflammatory markers were assessed using ELISA, whilst qRT-PCR studies were carried out for the mRNA expression of IL-1b, IL-6, RANKL, OPG, TNF-α and COX-2 genes. GCMS and HPLC analysis were performed to identify the secondary metabolites of
A. dumosus
. From day 8 to 28 post-administration of formaldehyde and CFA, oral administration of
A. dumosus
(600, 300 and 150 mg/kg) showed a noteworthy improvement (
p
< 0.001) in the body weights, immune organ weights, serum levels of rheumatoid (RA) factor, C-reactive protein, TNF-α and IL-6 levels in arthritic rats similar to the effect of piroxicam and methotrexate. Subsequently, the administration of
A. dumosus
to formaldehyde and CFA-challenged rats, caused a marked decrease (
p
< 0.001) in the mRNA expression of IL-1b, IL-6, OPG, RANKL, TNF-α and COX-2 genes in treated rats. Likewise, when assessed for antioxidant potential,
A. dumosus
produced a pronounced (
p
< 0.001) reduction in malondialdehyde (MDA) levels and hydrogen peroxide (H
2
O
2
) production, whilst a dose-dependent (
p
< 0.001) increase in catalase (CAT) and superoxide dismutase (SOD) activities was recorded. GCMS profiling of
A. dumosus
presented benzaldehyde, 3-hydroxy-4-methoxy-, 1-decanol and undecane as plant compositions, whereas HPLC fingerprinting displayed quercetin, benzaldehyde, 3-hydroxy-4-methoxy-, gallic acid and cinnamic acid as plants constituents. These results depict that
A. dumosus
possesses anti-arthritic effect mediated possibly through attenuation of arthritic indices, chronic inflammatory and oxidative stress biomarkers along with down-regulation in the mRNA expression of arthritic candid genes.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>38057565</pmid><doi>10.1007/s10787-023-01391-x</doi><tpages>23</tpages><orcidid>https://orcid.org/0000-0002-9959-1458</orcidid></addata></record> |
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| ispartof | Inflammopharmacology, 2024-02, Vol.32 (1), p.825-847 |
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| source | Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List |
| subjects | Allergology Animals Arthritis Benzaldehydes Biomarkers Biomedical and Life Sciences Biomedicine Cyclooxygenase 2 - genetics Dermatology Formaldehyde Freund's Adjuvant Gastroenterology Hydrogen Peroxide Immunology Interleukin-6 Original Article Oxidative Stress Pharmacology/Toxicology Rats Rheumatology RNA, Messenger - genetics Tumor Necrosis Factor-alpha - genetics |
| title | Assessment of antiarthritic potential of Asparagus dumosus using formaldehyde and CFA-induced arthritic models in rats via modulation of oxidative stress biomarkers and mRNA expression of IL-1b, IL-6, RANKL, OPG, TNF-α and COX-2 |
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