Separation of spring viraemia of carp virus from large-volume samples using immunomagnetic beads

A method for separation of spring viraemia of carp virus (SVCV) from large-volume samples using immunomagnetic beads (IMBs) coated with a polyclonal antibody against SVCV was developed. The optimum amount of IMBs was 2 mg in 100 mL. After IMB treatment, the detection limit of SVCV in reverse transcr...

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Bibliographic Details
Published in:Archives of virology 2024-01, Vol.169 (1), p.8-8, Article 8
Main Authors: Hongli, Jing, Min, Zhang, Longying, Gao, Liegang, Si, Na, Wang, Mingfeng, Ge, Shengwei, Xu, Jianping, Wang, Shaoqiang, Wu
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Brief Report
Carp
Carps
Fish Diseases
Immunomagnetic Separation
Infectious Diseases
Medical Microbiology
Pathogens
Plasmids
Polyclonal antibodies
Ponds
Reverse transcription
Rhabdoviridae - genetics
Rhabdoviridae Infections - veterinary
Tissue culture
Viremia
Virology
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Summary:A method for separation of spring viraemia of carp virus (SVCV) from large-volume samples using immunomagnetic beads (IMBs) coated with a polyclonal antibody against SVCV was developed. The optimum amount of IMBs was 2 mg in 100 mL. After IMB treatment, the detection limit of SVCV in reverse transcription quantitative PCR (RT-qPCR) was 10 3 times the 50% tissue culture infectious dose per mL in 100-mL samples. The concentration of viral RNA extracted from SVCV that had been separated using IMBs was 5.18 × 10 3 -fold higher than that of the unseparated SVCV. When fish samples were tested, the concordance rates of the IMBs/RT-qPCR and RT-qPCR were 100% and 67.5%, respectively.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-023-05927-y