Revealing the role of CCoAOMT1: fine-tuning bHLH transcription factors for optimal anther development

• Published in: Science China. Life sciences 2024-03, Vol.67 (3), p.565-578
• Main Authors: Lai, Zesen, Wang, Jianzheng, Fu, Ying, Wang, Menghan, Ma, Hong, Peng, Shiqing, Chang, Fang
• Format: Article
• Language: English
• Subjects: Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Basic Helix-Loop-Helix Transcription Factors - genetics; Basic Helix-Loop-Helix Transcription Factors - metabolism; Biomedical and Life Sciences; Coenzyme A; Cytosol; Fertility; Flowers; Gene expression; Gene Expression Regulation, Plant; Helix-loop-helix proteins (basic); Homeostasis; Life Sciences; Localization; Methyltransferase; Methyltransferases - genetics; Mutants; Nuclear transport; Phenotypes; Plant Proteins - genetics; Plant Proteins - metabolism; Pollen; Protein transport; Research Paper; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Transgenic plants
• ISSN: 1674-7305; 1869-1889
• DOI: 10.1007/s11427-023-2461-0

The tapetum, a crucial innermost layer encompassing male reproductive cells within the anther wall, plays a pivotal role in normal pollen development. The transcription factors (TFs) bHLH010/089/091 redundantly facilitate the rapid nuclear accumulation of DYSFUNCTIONAL TAPETUM 1 , a gatekeeper TF in the tapetum. Nevertheless, the regulatory mechanisms governing the activity of bHLH010/089/091 remain unknown. In this study, we reveal that caffeoyl coenzyme A O-methyltransferase 1 (CCoAOMT1) is a negative regulator affecting the nuclear localization and function of bHLH010 and bHLH089, probably through their K259 site. Our findings underscore that CCoAOMT1 promotes the nuclear export and degradation of bHLH010 and bHLH089. Intriguingly, elevated CCoAOMT1 expression resulted in defective pollen development, mirroring the phenotype observed in bhlh010 bhlh089 mutants. Moreover, our investigation revealed that the K259A mutation in the bHLH089 protein disrupted its translocation from the nucleus to the cytosol and impeded its degradation induced by CCoAOMT1. Importantly, transgenic plants with the probHLH089::bHLH089 K259A construct failed to rescue proper pollen development or gene expression in bhlh010 bhlh089 mutants. Collectively, these findings emphasize the need to maintain balanced TF homeostasis for male fertility. They firmly establish CCoAOMT1 as a pivotal regulator that is instrumental in achieving equilibrium between the induction of the tapetum transcriptional network and ensuring appropriate anther development.