Profiling the antidiabetic potential of GC-MS compounds identified from the methanolic extract of Spilanthes filicaulis : experimental and computational insight

• Published in: Journal of biomolecular structure & dynamics 2023-12, p.1-22
• Main Authors: Ojo, Oluwafemi Adeleke, Ogunlakin, Akingbolabo Daniel, Gyebi, Gideon Ampoma, Ayokunle, Damilare IyinKristi, Odugbemi, Adeshina Isaiah, Babatunde, Dare Ezekiel, Akintunde, Emmanuel Adewuni, Ezea, Samson Chukwuemeka, Asogwa, Nnaemeka Tobechukwu, Asaleye, Rotdelmwa Maimako, Ojo, Adebola Busola
• Format: Article
• Language: English
• ISSN: 0739-1102; 1538-0254
• DOI: 10.1080/07391102.2023.2291828

This study examines the nutritional composition, phytochemical profiling, and antioxidant, antidiabetic, and anti-inflammatory potential of a methanolic extract of leaves (MESFL) , , and studies. antioxidant, antidiabetic, and anti-inflammatory activities were examined. In the study, liver tissues were subjected to FeSO -induced oxidative damage and treated with varying concentrations of MESFL. MESFL contains a reasonable amount of nitrogen-free extract, moisture, ash content, crude protein, and fat, with a lesser amount of crude fiber. According to GC-MS analysis, MESFL contains ten compounds, the most abundant of which are 13-octadecenal and Ar-tumerone. In this study, MESFL demonstrated anti-inflammatory activities membrane stabilizing properties, proteinase inhibition, and inhibition of protein denaturation (IC = 72.75 ± 11.06 µg/mL). MESFL also strongly inhibited both α-amylase (IC = 307.02 ± 4.25 µg/mL) and α-glucosidase (IC = 215.51 ± 0.47 µg/mL) activities. Our findings also showed that FeSO -induced tissue damage decreased the levels of GSH, SOD, and CAT activities while increasing the levels of MDA. In contrast, treatment with MESFL helped to restore these parameters to near-normal levels, which signifies that MESFL has great potential to address complications from oxidative stress. Furthermore, the interaction of the GCMS-identified phytochemicals with the active sites of α-amylase and α-glucosidase molecular and ensembled-based docking displayed strong binding affinities of Ar-tumerone and 4-hydroxy-3-methylacetophenone to α-amylase and α-glucosidase, respectively. Taken together, the biological activities of MESFL might be a result of the effects of these secondary metabolites.Communicated by Ramaswamy H. Sarma.