Prevalence and distribution pattern of AmpC β-lactamases in ESBL producing clinical isolates of Klebsiella spp. in parts of Assam, India

• Published in: World journal of microbiology & biotechnology 2024-01, Vol.40 (1), p.38-38, Article 38
• Main Authors: Gogoi, Indrani, Saikia, Shyamalima, Sharma, Mohan, Onyango, Amos Oloo, Puzari, Minakshi, Chetia, Pankaj
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Applied Microbiology; Bacterial Proteins - genetics; beta-Lactamases - genetics; Biochemistry; Biomedical and Life Sciences; Biotechnology; Clinical isolates; Drug resistance; Environmental Engineering/Biotechnology; Genotype & phenotype; Genotypes; Humans; Klebsiella; Klebsiella - genetics; Klebsiella pneumoniae - genetics; Life Sciences; Microbial Sensitivity Tests; Microbiology; Multidrug resistance; Polymerase chain reaction; Prevalence; Public health; β Lactamase
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-023-03846-3

The production of extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamases is the most common explanation of multidrug resistance in clinical isolates of Klebsiella spp. In the present study, a total of 160 isolates of Klebsiella spp. were procured from the DBT-NER project with ethical clearance no. DU/Dib/ECBHR(Human)/2021-22/02). These were collected from various health settings of Assam and identified as drug-resistant. The isolates were screened for antibiotic susceptibility and phenotypic tests were performed on multidrug resistant isolates to confirm ESBL and AmpC β-lactamases production. The distribution pattern of ESBL and AmpC β-lactamase genotype was investigated by polymerase chain reaction (PCR). The results showed that among 107 multidrug-resistant (MDR) isolates of Klebsiella spp., 67.28% of isolates were ESBL producers and 56.07% were potential AmpC producers. The PCR results revealed that bla CTX−M was the most prevalent ESBL genotype. Among the ESBL producers, 11.11% of isolates showed co-occurrence with plasmid-mediated AmpC β lactamases genotype which indicated the high prevalence of ESBL and AmpC co-producers in K. pneumoniae and K. oxytoca , suggesting the possibility of serious public health concerns. Therefore, it is crucial to regularly monitor the spread of multidrug resistance among clinical isolates.