A monomeric StayGold fluorescent protein

StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. Despite favorable fluorescence properties, use of StayGold as a fluorescent tag is limited because it forms a natural dimer. Here we report the 1.6 Å structure of StayGold and generate a de...

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Bibliographic Details
Published in:Nature biotechnology 2024-09, Vol.42 (9), p.1368-1371
Main Authors: Ivorra-Molla, Esther, Akhuli, Dipayan, McAndrew, Martin B. L., Scott, William, Kumar, Lokesh, Palani, Saravanan, Mishima, Masanori, Crow, Allister, Balasubramanian, Mohan K.
Format: Article
Language:English
Subjects:
631/1647/245/2225
631/535/1266
Agriculture
Bioinformatics
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Brief Communication
Chloride
Chromatography
Crystal structure
Fluorescence
Interfaces
Life Sciences
Medical research
Molecular structure
Molecular weight
Mutagenesis
Mutation
Photobleaching
Protein structure
Proteins
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Summary:StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. Despite favorable fluorescence properties, use of StayGold as a fluorescent tag is limited because it forms a natural dimer. Here we report the 1.6 Å structure of StayGold and generate a derivative, mStayGold, that retains the brightness and photostability of the original protein while being fully monomeric. The bright, photostable fluorescent protein StayGold is converted into a monomer.
ISSN:1087-0156
1546-1696
1546-1696
DOI:10.1038/s41587-023-02018-w