Advances and applications of microcrystal electron diffraction (MicroED)

Microcrystal electron diffraction, commonly referred to as MicroED, has become a powerful tool for high-resolution structure determination. The method makes use of cryogenic transmission electron microscopes to collect electron diffraction data from crystals that are several orders of magnitude smal...

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Bibliographic Details
Published in:Current opinion in structural biology 2024-02, Vol.84, p.102741, Article 102741
Main Authors: Haymaker, Alison, Nannenga, Brent L.
Format: Article
Language:English
Subjects:
Cryo-electron microscopy
Cryo-EM
Cryoelectron Microscopy - methods
Crystallography
Crystallography - methods
Electrons
Membrane Proteins
MicoED
Microcrystal electron diffraction
Protein crystallography
Small molecule crystallography
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Summary:Microcrystal electron diffraction, commonly referred to as MicroED, has become a powerful tool for high-resolution structure determination. The method makes use of cryogenic transmission electron microscopes to collect electron diffraction data from crystals that are several orders of magnitude smaller than those used by other conventional diffraction techniques. MicroED has been used on a variety of samples including soluble proteins, membrane proteins, small organic molecules, and materials. Here we will review the MicroED method and highlight recent advancements to the methodology, as well as describe applications of MicroED within the fields of structural biology and chemical crystallography.
ISSN:0959-440X
1879-033X
1879-033X
DOI:10.1016/j.sbi.2023.102741