Functional characterization of four antenna-biased chemosensory proteins in Dioryctria abietella reveals a broadly tuned olfactory DabiCSP1 and its key residues in ligand-binding

The orientation of the oligophagous cone-feeding moth Dioryctria abietella (Lepidoptera: Pyralidae) to host plants primarily relies on olfactory-related proteins, particularly those candidates highly expressed in antennae. Here, through a combination of expression profile, ligand-binding assay, mole...

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Published in:Pesticide biochemistry and physiology 2023-12, Vol.197, p.105678-105678, Article 105678
Main Authors: Yin, Ning-Na, Yao, Yu-Juan, Liang, Yin-Lan, Wang, Zheng-Quan, Li, Yong-He, Liu, Nai-Yong
Format: Article
Language:English
Subjects:
Chemosensory protein
Dioryctria abietella
Ligand-binding property
Olfaction
Site-directed mutagenesis
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Summary:The orientation of the oligophagous cone-feeding moth Dioryctria abietella (Lepidoptera: Pyralidae) to host plants primarily relies on olfactory-related proteins, particularly those candidates highly expressed in antennae. Here, through a combination of expression profile, ligand-binding assay, molecular docking and site-directed mutagenesis strategies, we characterized the chemosensory protein (CSP) gene family in D. abietella. Quantitative real-time PCR (qPCR) analyses revealed the detectable expression of all 22 DabiCSPs in the antennae, of which seven genes were significantly enriched in this tissue. In addition, the majority of the genes (19/22 relatives) had the expression in at least one reproductive tissue. In the interactions of four antenna-dominant DabiCSPs and different chemical classes, DabiCSP1 was broadly tuned to 27 plant-derived odors, three man-made insecticides and one herbicide with high affinities (Ki 
ISSN:0048-3575
1095-9939
DOI:10.1016/j.pestbp.2023.105678