CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding

CRISPR (short for "Clustered Regularly Interspaced Short Palindromic Repeats") is a technology that research scientists use to selectively modify the DNA of living organisms. CRISPR was adapted for use in the laboratory from the naturally occurring genome-editing systems found in bacteria....

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Bibliographic Details
Published in:International journal of molecular sciences 2023-12, Vol.24 (23), p.16656
Main Authors: Martín-Valmaseda, Marina, Devin, Sama Rahimi, Ortuño-Hernández, Germán, Pérez-Caselles, Cristian, Mahdavi, Sayyed Mohammad Ehsan, Bujdoso, Geza, Salazar, Juan Alfonso, Martínez-Gómez, Pedro, Alburquerque, Nuria
Format: Article
Language:English
Subjects:
Abiotic stress
Adaptation
Agricultural production
CRISPR
CRISPR-Cas Systems - genetics
Crop diseases
Crops, Agricultural - genetics
Efficiency
Fruit
Fruit - genetics
Gene Editing - methods
Genes
Genetic engineering
Genetic research
Genome editing
Genome, Plant
Genomes
Genomics
Plant Breeding - methods
Proteins
Trees - genetics
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Summary:CRISPR (short for "Clustered Regularly Interspaced Short Palindromic Repeats") is a technology that research scientists use to selectively modify the DNA of living organisms. CRISPR was adapted for use in the laboratory from the naturally occurring genome-editing systems found in bacteria. In this work, we reviewed the methods used to introduce CRISPR/Cas-mediated genome editing into fruit species, as well as the impacts of the application of this technology to activate and knock out target genes in different fruit tree species, including on tree development, yield, fruit quality, and tolerance to biotic and abiotic stresses. The application of this gene-editing technology could allow the development of new generations of fruit crops with improved traits by targeting different genetic segments or even could facilitate the introduction of traits into elite cultivars without changing other traits. However, currently, the scarcity of efficient regeneration and transformation protocols in some species, the fact that many of those procedures are genotype-dependent, and the convenience of segregating the transgenic parts of the CRISPR system represent the main handicaps limiting the potential of genetic editing techniques for fruit trees. Finally, the latest news on the legislation and regulations about the use of plants modified using CRISPR/Cas systems has been also discussed.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms242316656