MdMYB66 Is Associated with Anthocyanin Biosynthesis via the Activation of the MdF3H Promoter in the Fruit Skin of an Apple Bud Mutant

Skin color is an important trait that is mainly determined by the content and composition of anthocyanins in apples. In this study, a new bud mutant (RM) from 'Oregon Spur II' (OS) of Red Delicious apple was obtained to reveal the mechanism underlying red color formation. Results showed th...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-12, Vol.24 (23), p.16871
Main Authors: Huang, Yaping, Li, Wenfang, Jiao, Shuzhen, Huang, Juanjuan, Chen, Baihong
Format: Article
Language:English
Subjects:
Anthocyanins - metabolism
Biosynthesis
Color
Enzymes
Flavonoids
Fruit - genetics
Fruit - metabolism
Fruits
Gene Expression Regulation, Plant
Horticulture
Malus - genetics
Malus - metabolism
Metabolism
Metabolites
Pigments
Plant Proteins - genetics
Plant Proteins - metabolism
Skin
Transcription factors
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Summary:Skin color is an important trait that is mainly determined by the content and composition of anthocyanins in apples. In this study, a new bud mutant (RM) from 'Oregon Spur II' (OS) of Red Delicious apple was obtained to reveal the mechanism underlying red color formation. Results showed that the total anthocyanin content in RM was significantly higher than that in OS with the development of fruit. Through widely-targeted metabolomics, we found that cyanidin-3-O-galactoside was significantly accumulated in the fruit skin of RM. Transcriptome analysis revealed that the structural gene and transcription factor were significantly up-regulated in the mutant. Overexpression of in apple fruit and apple callus significantly promoted anthocyanin accumulation and significantly increased the expression level of and structural genes related to anthocyanin synthesis. Y1H and LUC analysis verified that could specifically bind to the promoter of . The results of the double luciferase activity test showed that activated 3.8 times, which led to increased anthocyanin contents. This might explain the phenotype of red color in RM at the early stage. Taken together, these results suggested that was involved in regulating the anthocyanin metabolic pathways through precise regulation of gene expression. The functional characterization of provides insight into the biosynthesis and regulation of anthocyanins.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms242316871