Simultaneous detection of three nitrofuran antibiotics by the lateral flow immunoassay based on europium nanoparticles in aquatic products

•The EuNPs-mAb probes can recognize the AOZ, AMOZ and NFS in aquatic products.•A EuNPs-mLFIA was established for rapid quantitative of three NF antibiotics.•The multiple testing were completed within 10 min with high sensitivity.•The proposed method was suitable for promotion in remote and underdeve...

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Bibliographic Details
Published in:Food chemistry 2024-05, Vol.439, p.138171-138171, Article 138171
Main Authors: Mei, Qing, Ma, Biao, Li, Jiali, Deng, Xin, Shuai, Jiangbing, Zhou, Yuxin, Zhang, Mingzhou
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - analysis
aquaculture
Aquatic products
carcinogenicity
Europium
Europium nanoparticles
food chemistry
food safety
half life
Immunoassay
immunoassays
Lateral flow immunoassay
liquid chromatography
Metal Nanoparticles
Multiple detection
mutagenicity
nanoparticles
Nitrofuran metabolites
Nitrofurans - analysis
sodium
tandem mass spectrometry
Tandem Mass Spectrometry - methods
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Summary:•The EuNPs-mAb probes can recognize the AOZ, AMOZ and NFS in aquatic products.•A EuNPs-mLFIA was established for rapid quantitative of three NF antibiotics.•The multiple testing were completed within 10 min with high sensitivity.•The proposed method was suitable for promotion in remote and underdeveloped areas. Nitrofuran (NF) antibiotics have been banned worldwide in aquaculture due to their potential carcinogenicity and mutagenicity. Because of the short half-life of NF antibiotics, an easy and sensitive multiple lateral flow immunoassay (mLFIA) based on europium nanoparticles (EuNPs) has been successfully established to simultaneously and quantitatively detect 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), 3-amino-2-oxazolidinone (AOZ) and sodium nifurstylenate (NFS) in aquatic products. The EuNP-mLFIA assay was accomplished within 10 min. The limits of detection (LODs) for AOZ, AMOZ and NFS were 0.013, 0.019 and 0.023 ng/mL, respectively. The average recoveries of AOZ, AMOZ and NFS were 98.0–104.4%, 96.0–102.6% and 98.0–102.8%, respectively. It showed satisfactory consistency, and the feasibility was validated by high-performance liquid chromatography-tandem mass spectrometry (HPLC–MS/MS). Briefly, this method will become a powerful tool for monitoring multiple NF antibiotics and provide promising applications in the field of food safety and environmental testing.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2023.138171