Preparation of Novel Sea Cucumber Intestinal Peptides to Promote Tibial Fracture Healing in Mice by Inducing Differentiation of Hypertrophic Chondrocytes to the Osteoblast Lineage

• Published in: Molecular nutrition & food research 2024-01, Vol.68 (2), p.e2300344-n/a
• Main Authors: Yin, Haowen, Yue, Hao, Wang, Meng, Zhang, Tianqi, Zhao, Yun‐Tao, Liu, Hongying, Wang, Jingfeng, Zheng, Hongwei, Xue, Changhu
• Format: Article
• Language: English
• Subjects: Acetylation; Animals; Apoptosis; Bone healing; Callus; cartilage callus; Cell Differentiation; Chondrocytes; Chondrocytes - metabolism; Chondrogenesis; Differentiation; DNA methylation; Endochondral bone; Eutrophication; Fracture Healing - physiology; Fractures; Functional foods & nutraceuticals; Healing; Histones; Histones - metabolism; hypertrophic chondrocytes; Intestine; Mice; Mineralization; Ossification; Osteoblastogenesis; Osteoblasts; Osteoblasts - metabolism; Osteogenesis - physiology; Peptides; Peptides - metabolism; Peptides - pharmacology; Pluripotency; Raw materials; sea cucumber intestinal peptides; Sea Cucumbers; Tibia; tibial fracture; Tibial Fractures - metabolism; Transcription factors
• ISSN: 1613-4125; 1613-4133
• DOI: 10.1002/mnfr.202300344

Scope Hypertrophic chondrocytes have a decisive regulatory role in the process of fracture healing, and the fate of hypertrophic chondrocytes is not only apoptosis. However, the mechanism of sea cucumber (Stichopus japonicus) intestinal peptide (SCIP) on fracture promotion is still unclear. This study aims to investigate the effect of sea cucumber intestinal peptide on the differentiation fate of hypertrophic chondrocytes in a mouse tibial fracture model. Methods and results Mice are subjected to open fractures of the right tibia to establish a tibial fracture model. The results exhibit that the SCIP intervention significantly promotes the mineralization of cartilage callus, decreases the expression of the hypertrophic chondrocyte marker Col X, and increases the expression of the osteoblast marker Col I. Mechanically, SCIP promotes tibial fracture healing by promoting histone acetylation and inhibiting histone methylation, thereby upregulating pluripotent transcription factors induced the differentiation of hypertrophic chondrocytes to the osteoblast lineage in a manner distinct from classical endochondral ossification. Conclusion This study is the first to report that SCIP can promote tibial fracture healing in mice by inducing the differentiation of hypertrophic chondrocytes to the osteoblast lineage. SCIP may be considered raw material for developing nutraceuticals to promote fracture healing. The effect of sea cucumber intestinal peptides (SCIP) on promoting fracture healing was investigated using a tibial fracture model. SCIP has been found to promote tibial fracture healing in mice by inducing differentiation of hypertrophic chondrocytes to the osteoblast lineage.