Investigation of the Mechanism of Membrane Potential Generation by Heme-Copper Respiratory Oxidases in a Real Time Mode

Heme-copper respiratory oxidases are highly efficient molecular machines. These membrane enzymes catalyze the final step of cellular respiration in eukaryotes and many prokaryotes: the transfer of electrons from cytochromes or quinols to molecular oxygen and oxygen reduction to water. The free energ...

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Published in:Biochemistry (Moscow) 2023-10, Vol.88 (10), p.1513-1527
Main Author: Siletsky, Sergei A.
Format: Article
Language:English
Subjects:
Amino acids
Analysis
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Copper
Copper converters
Cytochrome
Electrochemical potential
Electrochemistry
Enzymes
Eukaryotes
Free energy
Heme
Hydrogen bonds
Hydrogen ions
Kinetics
Life Sciences
Measurement methods
Membrane potential
Membrane potentials
Membranes
Microbiology
Mitochondria
Molecular machines
Oxidases
Oxygen
Prokaryotes
Proteins
Protons
Real time
Redox reactions
Review
Site-directed mutagenesis
Translocation
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creator Siletsky, Sergei A.
description Heme-copper respiratory oxidases are highly efficient molecular machines. These membrane enzymes catalyze the final step of cellular respiration in eukaryotes and many prokaryotes: the transfer of electrons from cytochromes or quinols to molecular oxygen and oxygen reduction to water. The free energy released in this redox reaction is converted by heme-copper respiratory oxidases into the transmembrane gradient of the electrochemical potential of hydrogen ions Δ μ H+ ). Heme-copper respiratory oxidases have a unique mechanism for generating Δ μ H + , namely, a redox-coupled proton pump. A combination of direct electrometric method for measuring the kinetics of membrane potential generation with the methods of prestationary kinetics and site-directed mutagenesis in the studies of heme-copper oxidases allows to obtain a unique information on the translocation of protons inside the proteins in real time. The review summarizes the data of studies employing time-resolved electrometry to decipher the mechanisms of functioning of these important bioenergetic enzymes.
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subjects Amino acids
Analysis
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Copper
Copper converters
Cytochrome
Electrochemical potential
Electrochemistry
Enzymes
Eukaryotes
Free energy
Heme
Hydrogen bonds
Hydrogen ions
Kinetics
Life Sciences
Measurement methods
Membrane potential
Membrane potentials
Membranes
Microbiology
Mitochondria
Molecular machines
Oxidases
Oxygen
Prokaryotes
Proteins
Protons
Real time
Redox reactions
Review
Site-directed mutagenesis
Translocation
title Investigation of the Mechanism of Membrane Potential Generation by Heme-Copper Respiratory Oxidases in a Real Time Mode
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