Biosynthesis, characterization, magnetic hyperthermia, and in vitro toxicity evaluation of quercetin-loaded magnetoliposome lipid bilayer hybrid system on MCF-7 breast cancer

• Published in: Biochimica et biophysica acta. General subjects 2024-03, Vol.1868 (3), p.130543-130543, Article 130543
• Main Authors: Elbeltagi, Shehab, Saeedi, Ahmad M, Eldin, Zienab E, Alfassam, Haifa E, Alharbi, Hanan M, Madkhali, Nawal, Shakor, Abo Bakr Abdel, El-Aal, Mohamed Abd
• Format: Article
• Language: English
• ISSN: 0304-4165; 1872-8006
• DOI: 10.1016/j.bbagen.2023.130543

Novel biocompatible and efficient hyperthermia (HT) treatment materials for breast cancer therapeutic have recently garnered significant attention, owing to their effective ablation of cancer cells and minimal damage to healthy cells. Magnetoliposome (MLs) offer multiple possibilities for utilize in cancer therapy, including smart drug delivery (SDD) mediated by alternating magnetic fields (AMF). In this work, magnesium ferrite (MgFe O ) encapsulated with liposomes lipid bilayer (MLs), Quercetin (Q)-loaded MgFe O @Liposomes (Q-MLs) nano-hybrid system were successfully synthesized for magnetic hyperthermia (MHT) and SDD applications. The hybrid system was well characterized using X-ray diffraction (XRD), Fourier transforms infrared spectroscopy (FT-IR), Energy dispersive X-ray (EDX), Vibrating sample magnetometer (VSM), Transmission electron microscope (TEM), and Zeta Potential (ZP). The characterization results confirmed the improving quercetin-loading on the MLs surface. TEM analysis indicated the synthesized MgFe O , MLs, and Q-MLs were spherical with an average size of 23.7, 35.5, and 329.5 nm, respectively. The VSM results revealed that the MgFe O exhibit excellent and effective saturation magnetization (M ) (40.5 emu/g). Quercetin drug loading and entrapment efficiency were found to be equal to 2.1 ± 0.1% and 42.3 ± 2.2%, respectively. The in-vitro Q release from MLs functionalized magnetite Q-loaded MLs was studied at 44.19% at pH 5.1 and 69.87% at pH 7.4, proving the Q-loading pH sensitivity. The MLs and Q-MLs hybrid system as MHT agents exhibit a Specific absorption rate (SAR) value of 197 and 205 W/g, respectively. Furthermore, the Q-MLs cytotoxicity was studied on the MCF-7 breast cancer cell line, and the results demonstrated that the Q-MLs have a high cytotoxicity effect compared to MLs and free Q.