Effect of Medicago sativa seed powder (Plus vitamin E vs. vitamin E alone) on semen analysis in men with idiopathic infertility: A double blind randomized clinical trial

• Published in: Journal of ethnopharmacology 2024-03, Vol.322, p.117606-117606, Article 117606
• Main Authors: Shahmirzadi, Azita Sadough, Shafi, Hamid, Shirafkan, Hoda, Memariani, Zahra, Gorji, Narjes, Moeini, Reihaneh
• Format: Article
• Language: English
• Subjects: Female; Humans; Infertility, Male - drug therapy; Male; Medicago sativa; Powders - therapeutic use; Pregnancy; Seeds; Semen Analysis; Sperm Count; Sperm Motility; Spermatozoa; Vitamin E - pharmacology; Vitamin E - therapeutic use
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2023.117606

Different parts of the alfalfa plant (Medicago sativa L.), especially its seeds, have been introduced as a semen quality enhancer in the folk medicine of different regions of Iran as well as in the traditional Persian medicine (PM) books. The seeds of this plant are also used in many combined medicines to treat male infertility in PM. This study was designed to investigate the effect of M. sativa seed powder plus vitamin E vs. vitamin E alone on semen analysis of men with idiopathic infertility. In this randomized clinical trial, 60 participants were randomly divided into two groups and then received M. sativa seed powder (6 g daily) or placebo for three months, as add-on to vitamin E (100 U). Results of semen analysis (sperm total count, motility, and morphology as well as semen volume) of these two groups and their changes were compared before and after treatment as the primary outcome. The number of pregnancies after one month and probable side effects were also assessed. After 12 weeks, sperm total count changed from 36.2 ± 21.8 × 10 /ml to 48.5 ± 19.1 × 10 /ml in M. sativa and from 39.5 ± 23.5 × 10 /ml to 41.2 ± 20.9 × 10 /ml in placebo, percentage of normal morphology changed from 1.8 ± 0.8% to 2.6 ± 1.2 % in M. sativa and from 2.0 ± 0.9% to 2.6 ± 1.2% in placebo and percentage of motile sperm changed from 36.5 ± 11.8 % to 39.7 ± 12.0% in M. sativa and from 39.3 ± 10.1 % to 38.1 ± 12.1% in placebo. The improvements in M. sativa group are significantly better than placebo group (P = 0.00, 0.01 and 0.04, respectively). However, semen volume decreased in both groups, but its changes were not significant. The number of pregnancies was four in the intervention and zero in placebo group. One case with abdominal bloating and one with respiratory allergy withdrew from the intervention group. No other adverse effect was reported. In this study, significant improvement was detected in sperm parameters (except semen volume) of men with idiopathic infertility in M. sativa plus vitamin E group in compare to vitamin E alone after 12 weeks. These findings suggest that the M. sativa, as a complementary therapy, may have a beneficial effect on semen quality. More clinical trials with larger sample size are needed.